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凋亡相关基因TNFAIP3、ENC1在人脑胶质瘤组织中的表达 被引量：3

Expression of Apoptosis-related genes TNFAIP3 and ENC1 in Human Brain Glioma
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摘要 目的 检测细胞凋亡相关基因TNFAIP3和ENC1在不同级别胶质瘤组织和正常脑组织中的表达。方法 提取组织总RNA,用半定量RT-PCR检测其中TNFAIP3和ENC1 mRNA的表达。结果 (1)同一患者肿瘤组织与其正常脑组织相比,TN-FAIP3基因表达上调,ENC1基因表达下调;(2)与正常脑组织相比,WHO Ⅱ和Ⅳ级肿瘤组TNFAIP3表达升高,WHO Ⅰ、Ⅱ和Ⅲ级肿瘤组ENC1表达下降。结论 RT-PCR结果与芯片检测结果基本相符;胶质瘤组织中TNFAIP3表达上调、ENC1表达下调,它们的异常表达可能参与了胶质瘤的恶性进展过程。 Objective To detect the expression of apoptosis-related genes TNFAIP3 and ENC1 in different grade glioma specimens and normal brain tissues. Methods Extracting total RNA of tissues, then detecting the expression of TNFAIP3 and ENC1 with semi-quantitative RT-PCR. Results (1) TNFAIP3 was up-regulated and ENC1 down-regulated in glioma tissue comparing with normal brain tissue of same individual. (2) Compared with normal brain tissues, TN-FAIP3 was increased in WHO Ⅱ and Ⅳ groups, and ENC1 was decreased in WHO Ⅰ, Ⅱ, and Ⅲ groups. Conclusions The results of gene expression in RT-PCR were similar with cDNA array. The abnormal expression of TNFAEP3 and ENC1 may be involved in malignant progression of glioma.
作者 王爱东 黄强 陈寒春 王飞 蒋滢 缪竞诚
机构地区 苏州大学附属二院神经外科暨脑肿瘤研究室 苏州大学医学院
出处 《中国血液流变学杂志》 CAS 2003年第3期210-213,共4页 Chinese Journal of Hemorheology
基金 国家自然科学基金(30070772)
关键词 凋亡相关基因 TNFAIP3 ENC1 人脑胶质瘤组织 表达 glioma apoptosis TNFAIP3 ENC1 RT-PCR
分类号 R73-3 [医药卫生—肿瘤] R739.41 [医药卫生—肿瘤]
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参考文献9

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二级参考文献7

共引文献4

同被引文献46

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中国血液流变学杂志
2003年 第3期

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