8-Br-cAMP和槲皮素对Eca-109细胞凋亡中P38和Caspase-3表达的影响

Expression of P38 and Caspase-3 involved in apoptosis of Eca-109 cells induced by 8-Br-cAMP and quercetin

目的 :探讨 8 Br cAMP和槲皮素对Eca 10 9细胞凋亡中P38、Caspase 3表达的影响。方法 :将贴壁培养的Eca 10 9细胞随机分为 3组 :①对照组 :只加DMEM(Sigma)培养液 (含体积分数 10 %胎牛血清 )培养 4 8h。②Br组 :终浓度为 2× 10 -5mol/L 8 Br cAMP的培养液培养 4 8h。③Q组 :终浓度为 4 3μmol/L槲皮素的培养液培养 4 8h。对以上 3组细胞以TUNEL法染色计数细胞凋亡率。以免疫组化方法观察 3组细胞的P38MAPK IR和Caspase 3 IR反应性。结果 :Br组及Q组细胞凋亡率高于对照组 ;Br组及Q组细胞的P38MAPK IR高于对照组 ;Br组及Q组细胞的Caspase 3 IR亦高于对照组。P38MAPK IR与Caspase 3 IR呈正相关。P均 <0 .0 5。结论 :P38MAPK及Caspase 3参与了 8 Br cAMP及槲皮素诱导的Eca 10

Aim:To study whether P38 MAPK and Caspase 3 involved in Eca 109 cell apoptosis.Method: The cultured Eca 109 cells were divided into 3 groups: C group: the cultured cells without Br or Q , Br group: the cultured cells with 8 Br cAMP for 48 h, and Q group: the cultured cells with quercetin for 48 h. The cell apoptotic percentage was enumerated in each group by using TUNEL method. The P38 MAPK IR and Caspase 3 IR were observed by immunohistochemistry.Results: The apoptotic percentage in both Br and Q groups was much higher than that in C group.Both P38 MAPK IR and Caspase 3 IR in both Br and Q groups were stronger than that in C group. There was a positive correlation between P38 MAPK IR and Caspase 3 IR.Conclusion:The results showed that the P38 MAPK and Caspase 3 could be involved in Eca 109 cell apoptosis induced by 8 Br cAMP and quercetin.