胃癌组织中SP与p53基因的表达及形态定量分析

Expression and morphological quantitative analysis of SP and p53 gene in gastric carcinoma and adjacent tissues

目的 :探讨胃肠激素SP和突变型p5 3基因在胃癌组织中表达的临床意义及其演变关系。并为胃癌预防和早期诊断及胃癌细胞是否存在内分泌功能提供形态学依据。方法 :采用S P免疫组织化学方法检测了 33例胃癌组织、17例癌旁病变组织和 13例正常胃粘膜组织中SP、p5 3基因的蛋白表达 ,并同时应用图像分析技术对SP、p5 3阳性细胞进行了形状因子、等效直径和异形指数等指标的测定。结果 :免疫组织化学法检测显示 :胃癌组织中SP阳性表达明显高于癌旁组织和正常胃粘膜组织 (P <0 .0 0 1)。癌旁组织较正常胃粘膜组织阳性率增加但差异无统计学意义 (P >0 .0 5 )。伴淋巴结转移者阳性率明显高于无淋巴结转移者 (P <0 .0 1)。胃癌组织中P5 3基因蛋白阳性表达率和阳性细胞密度均高于癌旁组织 (P <0 .0 5 )。正常胃粘膜组织未见表达。胃癌组织SP、p5 3两基因共同表达率为 36 .4 % (12 / 33)。图像分析结果显示 :胃癌组织SP阳性细胞面积上大于正常胃粘膜组织 (P <0 .0 2 ) ,而形状因子、异型指数在各组间差异均无统计学意义 (P >0 .0 5 )。胃癌和癌旁组织中p5 3细胞核在等效直径、形状因子和异型指数各参数差异有统计学意义 (P <0 .0 1～ 0 .0 0 1)。结论 :SP与p5 3基因高表达参与了胃癌的发生和发展。SP免疫阳性细胞并非?

Aim:To explore the expression of gastrointestinal hormone SP and mutation type p53 gene in gastric carcinoma tissues so as to provide morphological indication for prevention and early diagnosis of gastric carcinoma.Methods:By using S P immunohistochemistry and image analysis technique, the expression of SP and P53 protein and the equivalent diameter, figure factor, and hetermorphic index of SP and p53 positive cells in 33 gastric carcinoma tissues,17 adjacent tissues, and 13 normal gastric mucosa tissues were examined.Results:The result of immunohistochemistry showed that: the positive expression of SP in gastric carcinoma tissues was apparently higher than those in both adjacent tissues and normal gastric mucosa tissues ( P <0.001).There was no statistical significance in SP positive expression between adjacent tissues and normal gastric mucosa tissues ( P >0.05).SP positive expression rate in tissues from gastric carcinoma with lymph node metastasis was apparently higher than that without ( P <0.01). Positive cell expression rate and positive cell density of P53 protein in gastric carcinoma were higher than that of adjacent tissues( P <0.05). The expression of p53 protein in normal gastric mucosa were not observed. Common expression rate of SP and P53 were 36.4% (12/33). Image analysis indicated that SP positive cell square of gastric carcinoma was larger than that of normal gastric mucosa( P <0.02). But regarding to the figure factor and hetermorphic index of each group there was no statistical significance( P >0.05). Equivalent diameter, shape factor, and hetermorphic index of P53 positive nuclear in gastric carcinoma were higher than that of adjacent tissues( P <0.01～0.001).Conclusions: Overexpression of SP and p53 genes takes part in the genesis and development of gastric carcinoma. SP positive cells are not all remained endocrine cells in gastric mucosa and some of them are carcinoma cells. Positive quantitative analysis of p53 could be the reference index for prevention and primary diagnosis of gastric carcinoma.