脆性组氨酸三联体基因异常转录与肝癌关系的研究

Relationship between aberrant FHIT transcripts and hepatocellular carcinoma

目的 研究脆性组氨酸二联体(FHIT)基因异常转录与肝细胞癌(HCC)的相关性。方法利用逆转录-聚合酶链反应(RT-PCR)和单链构象多态性(SSCP)技术检测24对肝癌及癌旁组织、4例正常肝组织中FHIT的转录和突变情况。结果 11例肝癌组织(46％)标本、2例(8％)癌旁正常组织检测到异常转录本,正常肝组织无异常转录。结论 FHIT基因异常转录可能是导致肝细胞癌形成的一个重要因素。

Objective To study the relationship between aberrant FHIT transcripts and hepatocellular carcinoma (HCC), Methods Reverse transcription-polymerase chain reaction (RT-PCR) and single strand conforma-tional polymorphism (SSCP) assays were used to analyze the transcripts and mutations of FHIT gene in 24 matched tumorous tissues and para-tumorous tissues from patients with HCC and in 4 normal liver tissues. Results Aberrant FHIT transcripts were observed in 11 out of 24 (46%) tumorous tissues and in 2 (8%) of the matched para-tumorous tissues. Conclusion FHIT aberrant transcripts may play an important role in the pathogenesis of hepatocellular carcinoma.