几种离子与人类精子顶体反应的关系

THE RELATIONSHIP BETWEEN SOME IONS AND ACROSOME REACTION OF HUMAN SPERM

本文用离子载体A23187诱导人类精子的顶体反应,并通过去除介质中Na^+,或在介质中加入K^+阻滞剂,或Ca^(2+)阻滞剂,或Na^+-K^+-ATP酶阻滞剂的方式来研究人类精了顶体反应与几种离子及Na^+泵的关系。结果表明:在人类精子顶体反应时,要有Na^+,K^+,Ca^(++)及Na^+泵的参与。但是,Ca^(++)进入精子的途径可能不是Ca^(++)的通道,

Calcium ionophore A 23187 has been used to induce acrosome reaction of human sperm and to investigate the relationship between some ions and acrosome reaction by removing Na^+ from the medium; by adding antagonist of K^+, TEA chloride; or antagonist of Ca^(++), Verapamil; or antagonist of Na^+-K^+-ATPase, acetyl stropharthidin. We have found that in acrosome reaction of human sperm, Na^+, K^+, Ca^(++) and Na^+-K^+-ATPase are required, and the rate of acrosome reaction can been enhanced by adding the antagonist of Ca^(++) (Verapamil). It seems that Ca^(++) ions are not entered by Ca^(++) channels.