肿瘤坏死因子α和血管紧张素Ⅱ对内皮细胞释放PAI-1的影响

The Effects of TNF-Alpha and Angiotensin Ⅱ on Endothelial Cell Secreting PAI-1

目的 研究肿瘤坏死因子α(TNF-α)和血管紧张素Ⅱ(Ang Ⅱ)对内皮细胞分泌组织纤溶酶原激活物(t-PA)和纤溶酶原激活物抑制剂-1(PAI-1)的影响。方法 培养内皮细胞株(ECV304),分为:(1)TNF-α刺激组,培养基中TNF-α加至终浓度为5、10、25、50、100ng/ml;(2)Ang Ⅱ刺激组,培养基中Ang Ⅱ加至终浓度为5、10、25、50、100ng/ml,24小时后测定上清中的组织纤溶酶原激活物(t-PA)和纤溶酶原激活物抑制剂-1(PAI-1)的含量。结果(1)空白对照组PAI-1含量为77.6±2.5ng/ml,TNF-α(5、10、25、50、100ng/ml)刺激组分别为80.5±0.9、89.2±6.9、81.1±1.1、86.7±5.6、100.9±11.0ng/ml,两组间差异显著(P<0.05),而两组t-PA含量无明显差异(P>0.05)。(2)空白对照组PAI-1含量为76.8±1.8ng/ml,Ang Ⅱ(50、100mnol/L)刺激组分别为79.2±0.9、80.1±1.2ng/ml,两组间差异显著(P<0.05),而两组t-PA含量无明显差异(P>0.05)。结论 TNF-α和Ang Ⅱ对内皮细胞株分泌的PAI-1有显著的升高作用,而对t-PA无显著影响。

Objective To investigate the effects of tumor necrosis factor Alpha (TNF-α) and angiotensin Ⅱ (Ang Ⅱ )on endothelial cell secreting tissue plasminogen activator(t-PA) and plasminogen activator inhibitor (PAI-1) .Methods Cultivated ECV304 cells were stimulated with TNF-a (5、 10、 25、 50、 100ng/ml) and Ang Ⅱ (5、 10、 25、50、 100nmol/L)for 24 hours. Tissue-type plasminogen activator (t-PA)and plasminogen activator inhibitor-1(PAI-1)in the supernatant of the cell culture were measured by enzyme linked immunosorbent assay (ELISA). Results The concentrations of t-PA had no statistically significant change, the ECV304 cells were stimulated by TNF-a or Ang Ⅱ . But PAI-1 was significandy increased by TNF-α (77.6±2.5ng/ml,versus 80.5 ±0.9±89.2±6.9±81.1 ± 1. 1±86.7 ± 5.6±100.9 ±11.0ng/ml), stimulated with TNF-α (5、 10、 25、 50、 100ng/ml). A distinct increase of PAI-1 was found (76. 8 ± 1.8ng/ml versus 79.2±0.9±80.1 ± 1.2ng/ml), stimulated with AngⅡ(50、10nmol/L). Conclusion TNF-α and Ang Ⅱ has no effects on t-PA released from ECV304. But, PAI-1 can be increased by TNF-α and Ang Ⅱ . TNF-α or Ang Ⅱ plays a role in the regulating of fibrinolysis.