NHL患者骨髓细胞DNA含量分析与患者临床生物学特性的关系

Correlation of DNA Content in Bone Marrow Cells and Clinical Biological Behavior of Patients with non-Hodgkins Lymphoma

目的 探讨NHL患者骨髓细胞DNA含量参数与患者临床生物学行为之间的关系。方法 采用流式细胞术对400例NHL患者骨髓细胞DNA指数、倍体类型、SPF和Apo进行了检测和分析。结果 NHL患者骨髓细胞的DI值、异倍体检出率、SPF均显著高于正常对照组(P<0.01)。NHL化疗者DI值和异倍体检出率显著低于未化疗者(P<0.05),而Apo却显著高于未化疗者(P<0.01)。随患者临床分期的增加,DNA异倍体检出率逐渐增加,Apo水平逐渐降低。结论 NHL能引起患者骨髓细胞生物学特性发生明显改变。而这些变化与患者临床生物学行为的关系十分密切。NHL患者骨髓细胞Apo水平变化可作为患者化疗效果和预后判断的客观指标。

Objective To study the relativity between DNA content parameters of bone marrow cells and clinical biological behavior of patients with non-Hodgkin's Lymphoma(NHL). Methods DNA index(DI), DNA ploid types, S-phase fraction (SPF), apoptosis(Apo) of bone marrow cells from 400 patients with NHL were detected by flow cytometry. Results The DI value, percentage of aneuploid and SPF in bone marrow cells were higher than those of normal controls (P <0.01). The DI value and percentage of aneuploid in patients with chemotherapy were lower than those of patients without chemotherapy (P < 0.05). Apo percentage in patients with chemotherapy was higher that of patients without chemotherapy (P < 0.01) . The percentage of aneuploid in bone marrow cells and the level of Apo increased gradually following the advancement of clinical stage. Conclusion There are significant changes of biological characteristic in bone marrow cells of patients with NHL, which are closely related to the clinical biological behavior in patients with NHL. The deletion of Apo level can provide objective evidence for therapy effect and prognosis of patients with NHL.