缺血性中风患者血浆载脂蛋白E与颈动脉内膜-中层厚度测定的临床意义

Detection of Apolipoprotein E and Carotid Intimamedia Thickness in the Ischemic Stroke and its Clinical Significance

目的 研究缺血性中风患者血浆载脂蛋白E(ApoE)水平与颈动脉内膜-中层厚度(IMT)测定的临床意义。方法 运用夹心ELISE法进行ApoE定量,应用多聚酶链反应(polymerise chain reaction,PCR)和限制性片断长度多态性方法分析(Restria-tion fragment length Polymorphism,RFLP)和彩色电脑声像仪,检测55例缺血性中风患者及50例正常健康体检者的载脂蛋白E的基因型和颈动脉内膜-中层厚度(IMT)。结果 缺血性中风患者携带ε_4异位基因的百分数为0.16(对照组0.05),ε_(2/4)基因型百分数为0.07(对照组0.02),ε_(3/4)基因型百分数为0.14(对照组0.08),两组对照,差异显著(P<0.05)。缺血性中风患者左、右两侧颈总动脉,分叉处,左、右颈内动脉IMT均显著高于对照组(P<0.01)。结论 血浆ApoE基因型含量升高和颈动脉IMT增厚可能是缺血性中风发生的危险因素的早期标志物。

To study the caroid intima-media thickness(IMT) in the ischemic stroke and its clinical significance. Methods Serum ApoE concentration were measured by ElASA , ApoE polymorphism was identified by polymerise chain reaction (PCR) and restriction fragment length polymorphism (KELP) and carotid intima-media thickness by color computer sonography from 51 subjects with ischemic stroke and 50 contral group (healthy physical examinee) .Results The frequency of genotype ε4, ε2/4 and ε3/4 respectively 0.16、0.07、 0.14, which was significantly higher than that in the control group(0.05、0.02、0.08)(P < 0.05);carotid intima-media thickness of ICCA,、RCCA 、LIMT、 RIMT , LICA and RICA was higher than CG sigmificantly ( P < 0.01). Conclusion The higer levels of serum ApoE concentration and caroid IMT may be early signs of risk factors in the occurrenal of ischemic stroke.