雷藤氯内酯醇与棉酚雄性抗生育作用的比较研究

COMPARATIVE STUDIES ON MALE ANTIFERTILITY EFFECTS OF TRIPCHLOROLIDE(T_4) AND GOSSYPOL

雷藤氯内酯醇(T_4)系从雷公藤中分离出来的具雄性抗生育作用的单体。棉酚为我国首创男用节育药,但存在少数服药者出现低血钾及停药后精子产生不可恢复现象,影响推广使用。本研究对T_4和棉酚的雄性抗生育作用及毒性进行比较,结果表明T_4作用环节较理想,主要作用在附睾精子,引起精子头尾大量分离,对睾丸曲细精管影响较小。因此在停药后能迅速恢复生育能力,而棉酚主要作用于曲精细管精母细胞阶段,所以恢复生育较慢,动物增重受阻,毒性较T_4显著。因此对T_4深入研究,可望发展成男用节育药。

The multiglycosides of Tripterygium wilfordii Hook. f. (GTW)has been shown to cause reversible infertility both in male rats and in men. Very recently, it was found that a new compound named tripchlorolide (T4) was isolated from GTW induced infertility in malerats, the potency of T4 being 200 times stronger than that of GTW. In the present study, we compared the antifertility effect and toxicology of T_4 and gossypol in male rats. Inhibition of spermatogenesis(spermatocytes) of rat testis by gossypol was more obvious than by T_4. Head and tail separation of epididymal spermatozoa in T_4 treated rats was more obvious than in gossypol treated rats. T_4 did not significantly affect body weight growth. It is concluded that T_4 may be developed into a male contraceptive.