何首乌对KA致大鼠脑胆碱能纤维损伤的保护作用

Protective effect of polygonum multiflorum thunb on the cerebral cholinergic neurofibers in rats

目的 :观察海人藻酸 (KA)对大鼠脑乙酰胆碱能神经元毁损后胆碱能 (AchE)纤维的数目和形态的改变 ,以探讨何首乌 (PMT)对胆碱能纤维的保护作用及机制。方法 :采用兴奋性神经毒素海人藻酸 (KA)损伤基底前脑 (BF)Meynert核团、内侧隔核 (MS)及斜角带核 (DB) ,建立毁损模型 ,用何首乌喂饲毁损实验组 ,并使用组织化学方法显示乙酰胆碱能纤维的改变及可能的用药后保护和活化作用。结果 :何首乌喂饲组与非何首乌喂饲组相比 ,其投射到海马及大脑皮质的AChE纤维数目多 ,纤维形态无破坏 ,差异有显著性 (P <0 .0 1)。结论 :何首乌对大鼠脑胆碱能神经投射纤维有保护作用。

Objective To observe the number and morphological changes of cholinergic neurofibers in rats under the destruction of kainic acid, and to investigate the protective effect and the mechnism of polygonum multiflorum thunb (PMT) on acetyl-chorine esterase (AChE) neurofibers. Methods Excitative neurotoxin kainic acid was injected into the basal forebrain, Meynert neucleus, medial septal neucleus and the Broca neucleus to estabolish the destruction model. Then the destructive experimental group was fed with PMT, and the histochemical method was used to display the changes of AChE fibers, the protection and activation of PMT. Results The number of projecting AChE fibers to the cortex and the hippocampus in PMT group was larger than that in the control group , and no morphological destruction was seen in the experimental group (P<0.01). Conclusion PMT has a protective effect on AchE projecting fibers in rats.