黄芪对新生儿脐血Th1/Th2功能平衡的影响

Effects of Astragalus Membranaceus on Functional States of Th1/Th2 in Cord Blood Mononuclear Cells of Neonates

目的 观察新生儿脐血单个核细胞 (CBMC) Th1/Th2的功能状态及黄芪 (AM)对新生儿 CBMC Th1/Th2功能平衡的影响。 方法 对 2 4例足月新生儿 CBMC体外分别经植物血凝素(PHA- P)和 PHA- P+AM刺激 (分为新生儿 PHA组和 AM组各 2 4例 ) ,培养 4 8h,用 EL ISA法检测培养上清液中白细胞介素 4 (IL - 4 )和γ干扰素 (IFN -γ)含量。以 15例正常儿童外周血单个核细胞(PBMC)为对照 (分为儿童 PHA组和 AM组各 15例 )。 结果 新生儿 PHA组 IFN-γ、IL - 4水平显著低于儿童 PHA组 (P均 <0 .0 1) ,IFN -γ/IL - 4比值也明显低于儿童 PHA组 (P<0 .0 5 ) ,与儿童比较呈现低水平的 Th2相对优势状态。新生儿 AM组 IFN -γ、IL - 4水平显著高于新生儿 PHA组 (P均 <0 .0 1) ,其中以 IFN-γ变化明显 ,IFN-γ/IL - 4比值明显高于 PHA组 (P<0 .0 5 )。儿童 AM组 IFN-γ水平较儿童 PHA组明显增高 (P<0 .0 1) ,IL - 4水平较 PHA组无明显变化 (P>0 .0 5 )。新生儿 AM组IFN-γ和 IL - 4水平明显高于儿童 PHA组 (P均 <0 .0 1) ,IFN-γ/IL - 4比值明显高于儿童 PHA组(P<0 .0 5 )。新生儿 AM组 IL - 4水平明显高于儿童 AM组 (P<0 .0 5 ) ,两组 IFN-γ水平差异无显著性(P>0 .0 5 ) ,IFN-γ/IL - 4比值明显低于儿童 AM组 (P<0 .0 1)?

Objective To observe the functional states of Th1/Th2 in cord blood mononuclear cells (CBMC) of neonates, and to study the effects of Astragalus Membranaceus(AM) on functional states of Th1/Th2 in CBMC. Methods Cord blood from 24 full-term neonates were drawn(divided into 24 neonatal PHA group and 24 neonatal AM group) and 15 healthy children controls (divided into 15 children's PHA group and 15 children's AM group)were assessed. After stimulation with PHA(PHA group) and PHA+AM(AM group) respectively, and cultured in vitro for 48 hours, the levels of IFN-γ and IL-4 in the supernatants of CBMC and peripheral blood mononuclear cells(PBMC) of children were detected by ELISA respectively. Results The levels of IFN-γ and IL-4 of neonatal PHA group were statistically lower than those of the children's PHA group(t=8.711,4.097;P< 0.01, respectively), and the ratio of IFN-γ/IL-4 (0.24±0.23) was lower than that of the controls(0.31±0.19;t=2.102, P<0.05). The levels of IFN-γand IL-4 of neonatal AM group were significantly higher than those of the neonatal PHA group (t=22.476,10.206; P<0.01, respectively), and the ratio of IFN-γ/IL-4 of AM group (0.36±0.15) was higher than that of the PHA group(t=2.204, P<0.05). The level of IFN-γ of children's AM group were markedly higher than that of the children's PHA group(t=15.182,P<0.01), but there was no difference in IL-4 level between two groups(t=0.653,P>0.05). The levels of IFN-γ and IL-4 of neonatal AM group were significantly higher than those of the children's PHA group(t=12.414,5.511;P<0.01, respectively), and the ratio of IFN-γ/IL-4 of neonatal AM group was higher than that of children's PHA group(t=2.171, P<0.05).The level of IL-4 of neonatal AM group was significantly higher than that of the children's AM group (t=2.672, P<0.05), and the ratio of IFN-γ/IL-4 of AM group was markedly higher than that of the children's AM group(0.75±0.26; t=8.71,P<0.01), but there was no difference in IFN-γ between two groups(t=0.612, P>0.05). Conclusions There is dysfunction of Th1/Th2 in neonates. The Th2 function slightly prevails over the Th1 function in neonates. The study suggests that the AM can enhance the Th1 function, reverse the enhanced Th2 function and improve the dysfunctional state in neonates. The AM can significantly improve the T-mediated specific immune activity in neonates. In conclusion, AM could be used to protect the infants from allergic diseases.