花药培养建立辣椒DH纯系的初步研究

Preliminary Studies on Establishing Doubled Haploid Lines of Pepper (Capsicum annuum L) by Anther Culture

试验结果表明 ,基因型是影响辣椒花药培养胚状体诱导率的主要因素 ,激素是影响辣椒胚状体诱导率的重要因素 ;0 .1mg/L的 2 ,4-D和 0 .1mg/L的KT配比适合大部分辣椒基因型成功出胚 ,且出胚率较高。在不同的时期接种 ,相同基因型胚状体的诱导率存在显著差异。添加活性炭能提高胚状体的诱导率 ,在 0 .2 5 %～ 0 .5 %的浓度区间内得到的诱胚率差异不明显。

The test in 2001-2003 shows that genotype is the main factor on the inducing rate of embryoid of pepper in anther culture.The combination of 2.4-D at 0.1 mg/L and KT at 0.1 mg/L is suitable for most peppers to induce embryoid and the inducing rate is quite high.There exists obvious differences on the inducing rate among different inoculating period for the embryoid with same genotype.Adding active carbon to medium will increase dearly the inducing rate of the embryoid,but there was no obvious diffence in the concentration of 0.25%-0.5%.