摘要
提取小麦DNA用4种不同的方法导入玉米植株,受体后代可产生6.09×10^(-4)～8.7×10^(-3)的变异株。变异性状多数为株高、生育期、株型、分蘖、育性等由多基因控制的数量性状。大部分变异性状随自交代数的增加而渐趋消失,有些性状在后代呈不规律的分离,只有少数变异性状可稳定遗传。本实验还对不同导入方法的转化效果进行了比较,若以变异率大小衡量,4种方法的依次顺序为花粉粒法>柱头法>籽粒注射法>生长点法。
After extracted, wheat DNA was introduced into maize plant with four different methods. Progeny of the recipient turned out to have number of maize mutants——6.09×10^(-4)×6.7×10^(-3). Characters of the mutants are mostly the quatitative characters, (which are controlled by polygenes), such as plant height, growing period, plant types, tiller and fertility. With the increase in seif-crosslng time, most of the variation characters, gradually disapeared. Some of the characters had irregular segregation in the self progeny, only few could be of hereditary stability. Conversion efficiencies of the different methods was compared in the experiment. The order from the highest to the lowest was: pollen grain introduction, stigma introduction, injecting DNA into kernel, growing point induction.
关键词
小麦
DNA
导入
玉米
Wheat DNA
Introduction
Maize
