摘要
公牛精囊、前列腺上皮内的分泌颗粒及腺腔内的分泌物中,含有带硫酸盐的糖蛋白,对精子的生长发育、成熟及生理活性有重要的作用。用 Schiff 氏高碘酸(PAS)法,阿尔生蓝(alcianblue·AB)法,可进行组织化学的定性分析。用强度不同的甲基化法可减弱 AB 反应的蓝色。而用 a-淀粉酶及 Schiff 氏高碘酸苯肼(PAPS)法的互相作用,可降低 PAS 反应的强度,以便观察腺体内糖蛋白的含量。
The Secretion granule in ox spermatangium and prostata epithelium andthe secretion in gland antrum containglycoproteins with sulfate,which takesan important part in thegrowth,development,mataration and physiologyactivity of sperm.The qualitative analysis of histochemistry could be doneby means of schiff's PAS and AB,The blue color in AB reaction wasreducedby methylation method which was different instrength,while,thestrength of PAS reaction was reduced by thecooperation of Schiff's PAPSin order that the glycoproteincontent in gland was observed.
关键词
公牛
精囊
前列腺
糖蛋白
组织化学
ox
spermatangium
prostata
glycoproteim
histochemistry
