洋葱染色体核型的FISH分析

Karyotype Analysis of Allium cepa L. by Fluorescence in Situ Hybridization with Satellite DNA

挑选洋葱 (AlliumcepaL .) ( 2n =2x =16 ,CC)有丝分裂中期细胞 ,以洋葱卫星DNA序列(AC SAT DNA)为探针 ,DIG 11 dUTP为标记物 ,用荧光原位杂交 (FISH)方法观察其在染色体上的定位 ,并进行洋葱的核型分析。 6号染色体为近端部着丝点染色体 ,在其长臂末端有一个卫星DNA序列的主要位点。其余每条染色体在其长、短臂的末端都各具有一个主要位点。差异表现在各染色体上卫星DNA序列的次要位点分布不同。根据洋葱 8对染色体各自具有的独特的卫星DNA分布位点 ,可将洋葱 8对染色体全部分开。以此为基础 。

In order to identify each pair chromosomes of Allium cepa L.,We used a simplified fluorescence in situ hybridization technique to localize the satellite DNA loci in metaphase cells of Allium cepa L.The resulting hybridization patterns enabled all onion chromosome pairs to be identified. Chromosome 6 C is an acrocentric chromosome.Unlike the others,it has only one main locus at the end of its long arm. Every pair of the whole chromosomes has its own special spotting pattern,by which the 8 pairs of onion chromosomes can be evidently identified.Thus,we made karyotype analysis of the genome.AC SAT DNA is a good probe for A cepa L.chromosome analysis.