NaCl胁迫下枸杞愈伤组织活性氧产生与质膜H^+-ATPase活性的关系

Relationship between active oxygen production and plasma membrane H^+-ATPase activity in calli of Lycium Barbarum under salt stress

以枸杞愈伤组织为材料,研究了盐胁迫对活性氧伤害和质膜H+-ATPase活性的影响。结果表明,NaCl浓度为100mmol/L时超氧阴离子(O2-·)和过氧化氢(H2O2)含量上升,质膜H+-ATPase活性先升后降;质膜相对透性与丙二醛(MDA)含量呈显著正相关;超氧阴离子(O2-·)和过氧化氢(H2O2)含量与质膜H+-ATPase活性在重度胁迫下呈显著负相关,说明盐胁迫下活性氧积累可能是加速伤害质膜功能的主要原因之一。

The experiment was conducted to study the effect of NaCl stress on plasma membrane H\++-ATPase activity and active oxygen damage in calli of Lycium barbarum. It was found that the content of superoxide radical (O2·) and hydrogen peroxide (H2O2) increased, while plasma membrane H+-ATPase activity increased under salt stress of 100 mmol/L but decreased under salt stress of 200~400 mmol/L. Membrane relative permeability was positively related to MDA. The content of O2· and H2O2 was negatively related to plasma membrane H\++-ATPase activity under 200~400 mmol/L NaCl stress. The results showed that the functional damage of plasma membrane in calli of Lycium barbarum under salt stress might be mainly causely by the accumulation of active oxygen.\;