核因子κB在佐剂性关节炎大鼠肺组织中的表达

Expression of NF-κB in the lungs of adjuvant arthritis rats

目的 研究核因子κB (NF κB)在佐剂性关节炎 (AA)大鼠肺组织中的表达 ,以探讨类风湿肺的发病机制。方法 通过给大鼠右后足足跖部皮下注射完全弗氏佐剂 (CFA)建立类风湿关节炎 (RA)的动物模型—AA。用蛋白质印迹 (Westernblot)法研究AA大鼠肺组织中活化NF κB蛋白水平 ,用原位杂交法进一步研究AA大鼠肺组织中NF κBmRNA水平 ,用显微图像分析系统对其表达进行定量分析。结果 在AA大鼠肺组织中可见肺泡变形 ,肺泡毛细血管基底膜损伤 ,间质内可见淋巴细胞、浆细胞及巨噬细胞浸润。AA组活化NF κB蛋白水平明显高于正常组 (P <0 0 1 ) ,NF κBmRNA的表达也明显高于正常组 (P <0 0 1 )。NF κBmRNA主要表达于肺内巨噬细胞、浆细胞以及浸入肺间质内的淋巴细胞中。结论 AA大鼠肺组织的病理改变与RA患者肺组织的病理改变相似 ,且NF

Objective To investigate the expression of NF κB in the lungs of adjuvant arthritis (AA) rats and to explore its possible role in the pathogenesis of rheumatoid arthritis (RA) pulmonary involvement.Methods The level of active NF κB protein was detected by Western blot and the level of NF κBmRNA by in situ hybridization histochemistry The intensity of expression was analyzed by micro image analysis system.Results The levels of active NF κB protein and NF κB mRNA in the lungs of AA rats were much higher than those of the controls ( P <0 01) NF κB mRNA was found mainly in the macrophages,plasmocytes and lymphocytes around the pulmonary arterioles.Conclusion The pathological changes of lung tissues in AA rats were similar to those of RA patients.NF κB maybe involved in the pathological changes of lung tissues in AA rats.