人参二醇对四氧化二氮染毒鼠α1-抗胰蛋白酶水平的影响

Effect of Panaxadiol on the α1-antitrypsin(α1-AT) of the mice with nitric tetroxide poisoning

目的 探讨人参二醇对四氧化二氮 (N2 O4)染毒鼠血清蛋白醇抑制物水平的影响 ,寻找简便易行的N2 O4中毒防治新途径。方法 LACA雄性小鼠 ,18～ 2 2g,共 12 3只。按体重随机分成对照组、染毒组、染毒加人参二醇组 ,每组 4 1只。模型制作 :对照组及染毒组每只小鼠均给予腹腔注射生理盐水 0 2mL ,人参二醇组每只小鼠给予腹腔注射人参二醇 0 2mL ,连续 7d。复制N2 O4急性中毒性肺水肿模型 ,中毒后 15min、1h分别腹腔内注射上述药物共 2次。注射完毕后观察小鼠的表现和存活情况。 7d后取眼球静脉血血清 ,- 2 0℃保存。测定血清α1-抗胰蛋白酶 (α1-AT)水平。结果 正常对照组小鼠经过腹腔注射人参二醇后 ,血清α1-AT水平无明显变化。而N2 O4染毒小鼠在伤后 1h ,与对照组比较 ,其血清α1-AT明显下降 ,在统计学上具有显著差异 (P <0 0 0 1)。人参二醇组小鼠在受伤后 1h ,其血浆α1-AT也有不同程度的下降 ,但下降程度明显低于单纯N2 O4组 (P <0 0 0 1)。染毒后 4 8h ,对照组血清α1-AT水平与染毒组无明显差异 ,而人参二醇治疗组α1-AT水平较其他两组明显升高 ,具有显著统计学差异 (P <0 0 1)。结论 人参二醇能够减轻急性中毒性肺水肿小鼠血清α1-AT的下降。其具体机制尚有待于进一步研究。

Objective To investigate the effect of Panaxadiol on the α1-antitrypsin(α1-AT) levels of the mice with nitric tetroxide poisoning, and then to look for new method to deal of intoxication of nitric tetroxide. Methods One hundred and twenty-three male LACA mice were randomly divided into 3 groups according to body weight:control group, intoxication group, Panaxadiol group. To prepare the intoxication model, nitric tetroxide was given to mice by inhalation for 15 minutes. Panaxadiol was administrated by peritoneal injection (0.2 mL, 7 days) before intoxication, the serum was isolated from each mice and then the α1-AT was detected respectively. Results Intoxication of nitric tetroxide, comparring with control group, could reduce the α1-AT level significantly. Administration of Panaxadiol could not affect the normal α1-AT level of the control group but could ameliorate the decreased level of α1-AT of the intoxication group. Conclusion Pretreatment with Panaxadiol could reduce the increasing level of α1-AT which induced by nitric tetroxide. These beneficial effects of Panaxadiol may be due in part to the direct inhibitory effect on neutrophil elastase, in addition to the protection of the dysfunction of liver and kidney. [