用PCR和反杂交法对透明质酸在慢性牙周炎治疗中微生物学作用的研究

The study of the microbiological effects of hyaluronic acid gel in the treatment of chronic periodontitis by polymerase chain reaction and reverse hybridization assay.

目的 :观察外源性透明质酸局部应用对慢性牙周炎的微生物学作用。方法 :采用口内自身对照研究 ,选择慢性牙周炎患者 2 0例 ,受试牙 30 6个 ,进行常规洁治、刮治和根面平整 ,实验组龈下辅助给予 0 .2 %透明质酸(HA)凝胶。采用PCR和反杂交方法检测龈下菌斑中A .a .、P .g .、P .i.、B .f .和T .d .等五种牙周致病菌的动态变化。结果 :治疗后 ,对照组和实验组 5种牙周致病菌量的下降在统计学上无显著性。结论 :未发现HA对慢性牙周炎患者的牙周致病菌在体内有显著抑制作用。

Objective:Microbiological effects in local application of hyaluronic acid (HA) adjunctive to scaling and root planing (SRP) were evaluated.Method:In this cross over design study a total of 306 teeth of 20 patients with chronic periodontitis were included. All patients were treated with full mouth SRP, in addition a HA gel was administered subgingivally in the test site. Subgingival plaque samples were taken to determine the presence of A.a.,P.g.,P.i.,B.f., T.d. by polymerase chain reaction (PCR) and reverse hybridization assay.Result:The quantity of tested periodontal pathogens did not decrease significantly. Conclusion:No microbiological improvement could be achieved by the adjunctive use of HA gel to SRP.