复发性生殖器疱疹患者外周血趋化性细胞因子及其受体的研究

Detection of serum chemokines and their receptors on peripheral blood CD3^+T lymphocytes of patients with recurrent genital herpes

目的 :在单细胞水平上研究复发性生殖器疱疹 (RGH)外周血T淋巴细胞上CCR2、CCR5、CXCR1、CXCR3的表达 ,并通过观察血清趋化性细胞因子的改变 ,探讨趋化性细胞因子及其受体在RGH发病机理中的作用。方法 :采用ELISA法检测 2 0例RGH患者及 30例正常人血清中白细胞介素 8(IL 8)、γ干扰素诱导的蛋白 10 (IP 10 )、单核细胞趋化蛋白 1(MCP 1)、巨噬细胞炎性蛋白 12 (MIP 12 )活化后可调节的正常T细胞表达和分泌的因子 (RANTES) ,巨噬细胞来源的趋化性细胞因子(MDC)等水平变化 ;同时用双色荧光标记的流式细胞术分析 2 0例RGH患者外周血淋巴细胞表面趋化因子受体CCR2、CCR5、CXCR1、CXCR3的表达情况。结果 :RGH患者血清RANTES水平低于正常对照组 (P <0 0 5 ) ;外周血CXCR1+ T淋巴细胞百分率高于正常对照组 (P <0 0 5 )。结论 :病毒反复感染机体后 ,抑制机体的免疫细胞产生RANTES并通过T淋巴细胞CXCR1+ 的高表达可能在RGH抗病毒感染的免疫机理中起重要作用。

Objective:To study the role of serum chemokines and their receptors expression in pathogenesis of recurrent genital herpes(RGH).Methods:Serum levels of interleukin 8(IL 8),interferon γ inducible protein 10 (IP 10),monocyte chemotactic protein1(MCP 1),macrophage inflammatory protein 1α(MIP 1α),regulated upon activation,normal T cell expressed and secreted factor(RANTES) and macrophage derived chemokine(MDC)were determined by enzyme linked immunosorbent assay in 20 patients with RGH and 30 normal controls, meanwhile, a two color immunofluorescent staining of chemokine receptors CCR2,CCR5,CXCR1 and CXCR3 on peripheral blood CD3 +T lymphocytes of patients with RGH were analyzed by flow cytometer.Results:Serum levels of RANTES were lower in patients with RGH than that in controls(P<0 05).The number of CXCR1 positive T cells in perpheral blood was increased in patients with RGH compared to controls(P<0 05).Conclusion:Human various cells can be stimulated by herpes simplex virus 2 chronic and repeated infection to express higher CXCR1 + on CD3 +T cell and decrease the amount of RANTES.These results suggests that the immune response of antiviral infection play a very important role in the pathogenesis of RGH. [