期刊文献+

实时定量PCR研究进展及其应用 被引量:29

Research development and application of the real-time PCR
下载PDF
导出
出处 《中国预防兽医学报》 CAS CSCD 北大核心 2003年第5期395-399,共5页 Chinese Journal of Preventive Veterinary Medicine
基金 该项目为"中国科学院百人计划"资助项目
  • 相关文献

参考文献25

  • 1reaction analysis of cytokine and cytokine receptor gene expression during thymic ontogeny [J]. Cytokine,1997, 97:17 726.
  • 2Wall S J, Edwanl D R. Quantitative reverse transcription-polymerase chain reaction (TR-PCR): A comparison of prime-dropping, competitive, and real-time RT-PCRs[J]. Anal Biochem, 2002, 300 (2):269-273.
  • 3Willard M F, Stephen J W, Kent E V. Quantitative RT-PCR: Pitfall and Potential[J]. Bio Techniques, 1999, 26(1 ) : 112-125.
  • 4Giulietti A, Overbergh L, Valckx D, et al. An overview of real-time quantitative PCR: application to quantify cytoking gene expression[J]. Methods, 2001, 25(4):386-401.
  • 5Heid, C A, Stevens J, Livak J K, et al. Real time quantitative PCR[J]. Genome Res, 1996,6:986-994.
  • 6Wittwer C T, Hemnann M G, Moss A A, et al. Continuous fluorescence monitoring of rapid cycle DNA amplification [ J ]. Biotechniques, 1997, 22:130-138.
  • 7Woo T H, Patel B K, Cinco M, et al. Identification of Leptoepira biflexa by real-time homogeneous detection of rapid cycle PCR product[J]. Journal of Microbiological Methods, 1999, 35:23-30.
  • 8Chris H, Philippa L, Se´ verine T, et al. Mclting curve analysis of feline calicivirus isolates detected by real-time reverse transoription PCR [J]. Journal of Virological Methods, 2002, 106:241-244.
  • 9Tyagi S,Kramer F R. Molecular beacons: probes that fluoresce upon hybridization [J]. Nature Biotechnology, 1996, 14:303-308.
  • 10Whitcombe D, Theaker J, Guy S P, et al. Detection of PCR products using serf-probing amplicons and fluorescence [J]. Nature Biotechnol, 1999, 17: 804-807.

同被引文献399

引证文献29

二级引证文献197

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部