小偃麦异附加系的细胞学及分子标记鉴定

IDENTIFICATION OF WHEAT-THINOPYRUM INTERMEDIUM ALIEN ADDITION LINES USING CYTOLOGY AND MOLECULAR MARKERS

利用形态学、细胞学、A -PAGE和RAPD方法 ,对 5个小麦 -中间偃麦草 (Thinopyrumintermedium)双体异附加系Line5、Line6、Line11、Line12和Line13进行了鉴定。细胞学鉴定结果表明 ,它们根尖细胞染色体数目为 2n =4 4 ,花粉母细胞减数分裂中期Ⅰ (PMCMⅠ )染色体构型为 2n =2 2Ⅱ ,具有高度的细胞学稳定性 ;形态学鉴定和A-PAGE电泳分析证明 ,Line12和Line13可能附加了中间偃麦草第 2部分同源群的染色体 ,Line5和Line6可能附加了中间偃麦草第 1部分同源群的染色体 ;RAPD分析表明 ,在供试的 80个随机引物中 ,有 2个引物S2 0和S2 1能够在亲本中间偃麦草和双体异附加系中稳定扩增出特异带型 ,并可作为异附加系所附加染色体的特异RAPD标记。

wheat-Thinopyrum intermedium alien disomic addition lines (Line5、Line6、Line11、Line12 and Line13) were identified by using the methods of morphology、cytology、A-PAGE and RAPD analysis. Cytologic testing results showed that the number of chromosomes in their root tip cells was 2n=44 and the chromosome configuration at PMC MⅠwas 2n=22Ⅱ. Results of morphology and A-PAGE showed that Line12 and Line13 might contain Th.intermedium chromosomes of homoeologous group 2, the added alien chromosomes of Line5 and Line6 might belong to homoeologous group 1. 80 random primers were used for RAPD amplification, the specific bands of Th.intermedium parent could be observed in the electrophoretogram of 5 disomic addition lines by 2 of them, which could be used as RAPD markers for alien addition chromosomes.