影响草莓叶片植株再生因素的研究

ESTABLISHMENT OF TUDLA(FRAGARIAANANASSA) REGENERATION SYSTEM

目前建立草莓的再生体系大多采用TDZ作为主要激素 ,本研究以广泛栽培的四个草莓品种为材料 ,系统地研究了不同基因型、叶龄、褐化、暗培养、基本培养基、不同激素组合、Ag+ 等诸多因素对叶片再生的影响 ,建立了不依赖于TDZ的弗吉尼亚草莓叶片外植体的不定芽再生体系。研究结果表明Ag+ 并不能促进草莓弗吉尼亚植株的再生 ,反而起到了抑制作用。以顶部充分伸展的 18～ 2 1d叶龄的无菌苗叶片为外植体 ,再生效果最好 ,将此类叶片放在以MS为基本培养基、附加 1.6～ 1.8mg/L 6 -BA ,0 .1mg/LNAA ,0 .4 - 0 .5mg/LKT ,0 .1mg/L2 ,4 -D的分化培养基上 ,14d后便可见到不定芽 ,经过愈伤组织阶段从叶片上分化产生 ,出现的高峰期在接种后的 2 0～30d ,芽分化率高达 6 0 %以上。该再生体系可作为基因转化的受体系统。

The various factors affecting plant regeneration of several strawberiy cultivars were studied. The results showed that 6-BA and 2,4-D in plant initiation medium played an important role in improving plant regeneration if without TDZ. The most effective concentration for 6-BA and 2,4-D was 1.6-1.8mg/L, 0.1mg/L, respectively, which increased the regeneration frequency of Tudla(Fragariaananassa) up to 60%. The regeneration frequency of Tudla was decreased by adding AgNO 3 to the medium. In addition, different varieties(genotype), seedling age, MS medium, AgNO 3 etc. also affected plant regeneration from strawberry leaf.