胆酸、栀子苷及配伍对大鼠缺血再灌注脑组织TNF-α、IL-1β和ICAM-1含量的影响

Effect of Cholic Acid and Jasminoidin on Contents of TNF-α, IL-1β and ICAM-1 in Rat Brain with Injury of Cerebral Ischemia and Reperfusion

目的 :观察胆酸、栀子苷及两者配伍应用对局灶性脑缺血再灌注脑组织TNF -α、IL - 1β和ICAM - 1含量的影响 ,探讨中药对抗缺血再灌注损伤的作用机制。方法 :成年雄性SD大鼠 ,随机分成假手术对照组、模型组和给药组 (胆酸组、栀子苷组、合用组、血栓通组 )。采用大脑中动脉栓塞方法 ,制作缺血 2h再灌注 12h的局灶性脑缺血再灌注模型 ,应用放射免疫法 (RIA)检测脑组织匀浆TNF -α和IL - 1β含量 ,酶联免疫法 (ELISA)检测脑组织ICAM - 1含量。结果 :再灌注 12h后 ,脑组织TNF -α、IL - 1β和ICAM - 1蛋白量升高。胆酸可以明显降低TNF -α和IL - 1β含量 ,与模型组比较P <0 0 1和P <0 0 5 ,而不影响ICAM - 1含量。栀子苷对三者表达无明显影响。而两者合用可显著降低TNF -α和ICAM - 1含量 ,与模型组差异显著 (P <0 0 1) ;而合用对IL - 1β表达没有明显影响。结论 :胆酸降低致炎因子TNF -α表达的作用在和栀子苷配伍应用后作用更显著 ,而胆酸干预IL - 1β表达的作用在与栀子苷配伍后降低 ,两者合用产生了单独使用时均不具有的降低ICAM - 1的作用。据此推断 ,中药的配伍作用不是组分各自生物效应的简单累加 ,而表现为增效或减效的作用 ,配伍应用与药物单独使用作用机制可能不同。

Objective: To study and explore the effect of Cholic Acid and Jasminoidin and a combination of the two components on and anti the injury of focal cerebral ischemia and reperfusion by measuring TNF-α, IL-1β and ICAM-1 in rat brain with middle cerebral artery occlusion (MCAO). Method: Adult male SD rats were randomly divided into sham-operation group, 2h ischemia and 12h reperfusion model group and Chinese materia medica treatment group (Cholic Acid group, Jasminoidin group, the group of combination of Cholic Acid and Jasminoidin, and Xueshuan Tong group). The model group of 2h ischemia 12h reperfusion of focal cerebral ischemia and reperfusion were built up by MCAO. The contents of TNF-α, IL-1β in rat brain tissue were measured by Radioimmunoassay (RIA) and that of ICAM-1 was measured by Enzyme-linked immunoadsorbent assay (ELISA). Results: Compared to the sham-operation group, the model group showed that the contents of TNF-α, IL-1β and ICAM-1 significantly increased after 2h ischemia and 12h reperfusion. Compared to the model group , Cholic Acid reduced the expression of TNF-αand IL-1β markedly ( P <0.01 , P <0.05), but it had no effect on the expression of ICAM-1. Jasminoidin had no influence on the expression of all of them. TNF-αand ICAM-1 could be significantly decreased by Using Cholic Acid and Jasminoidin together ( P <0.01), but had no influence on the expression of IL-1β. Conclusion: Cholic Acid can reduce the expression of TNF-αand this effect is highly improved when combined with Jasminoidin. While, Cholic Acid's action of reducing the content of IL-1β can be decreased by using Cholic Acid and Jasminoidin together. They can significantly reduce ICAM-1, which neither of them can make it individually. Thus, it can be concluded that the mechanism of compatibility of Chinese medicines are not a simple pile-up of biological actions of the single drugs, and the compatibility of Chinese medicines might be different from when they are used individually.