摘要
以野生川梨为材料 ,采用溶液培养的方法 ,研究了缺铁胁迫对其生理的影响。结果表明 ,缺铁使川梨叶绿素a、叶绿素b和叶绿素总量明显降低 ;光合速率显著下降 ,而且光合速率的降低先于叶绿素含量的减少。川梨在介质供铁浓度低于 2 5 μmol·L-1时才发生失绿症状。在缺铁胁迫下 ,叶内POD和CAT活性降低 ,但降低幅度和时间不尽相同 ,而且CAT比POD与叶绿素的相关性更显著。缺铁叶内各营养元素含量高于正常供铁处理 ,并表现出不同的变化规律 ;活性铁分别与叶绿素、光合速率呈极显著正相关 ,可作为铁营养生理诊断指标。
A wild species of Pyrus, P. pashia Buch-Ham was used to investigate the physiological effects of iron deficiency in culture solution. The results showed that Chla, Chlb, total Chlorophyll content and photosynthesis rate(Pn) decreased sharply, and the decrease of Pn was prior to that of Chl content under iron deficiency. Iron deficiency symptoms occurred when its concentration in culture medium was less than 25 μmol·L -1 . Peroxidase(POD) and catalase(CAT) activity in Fe-deficient leaves declined significantly. POD was more sensitive than CAT to Fe deficiency. Howe-ver, the positive correlation between CAT activity and Chl content was more significant than that between POD activity and Chl content. The contents of nutrient elements in Fe-deficient leaves, which changed irregularly, were higher than that in normal leaves. There were a very significant positive correlation between active Fe and Chl content, and between active Fe and Pn respectively. Therefore, active Fe could be useful physiological predicting parameter for iron deficiency diagnosis.
出处
《中国农业科学》
CAS
CSCD
北大核心
2003年第8期935-940,共6页
Scientia Agricultura Sinica
