兔圆小囊产溶菌酶细胞的免疫电镜组织化学观察

Study on the Distribution of Lysozyme-producing Cells in Rabbit's Sacculus Rotundus with Immunohistochemistry and Immuno-electronmicroscopic Cytochemistry Methods

采用免疫组织化学和免疫电镜细胞化学的方法 ,对兔圆小囊组织中产溶菌酶细胞进行了观察。免疫组织化学观察发现 ,产溶菌酶阳性细胞在兔圆小囊的粘膜上皮、圆顶上皮以及淋巴组织的滤泡生发中心、圆顶区和帽区均有分布 ,且受到多杀性巴氏杆菌感染后 ,阳性细胞增多 ,阳性反应增强。免疫电镜结果显示 ,在兔圆小囊的淋巴组织DNES细胞的颗粒中发现溶菌酶免疫组织化学阳性反应物。这一重要发现为神经 内分泌 免疫网络学说提供了新的证据。

The expression of lysozyme in the tissue of rabbit's sacculus rotundus(RSR) (an immune-neuro-endocrine organ at the crossage of ileum and caecum) and its significance in intestinal mucosal immunity were studied. Immunohistochemistry and immuno-electron microscopic cytochemistry staining were used to detect lysozyme's expression in the RSR. Lysozyme-immuno-reactive-positive cells not only distrtbuted in mucosal epithelium and dome epithelium,but also in follicular geminal center, dome, and cap zone of the lymphoid tissue in RSR. Moreover, the cells that contained immuno-reactive substance increased and the extent of the reaction enhanced after the rabbits had been infected with Pasteurella multocida . The electron microscopic observation showed that lysozyme-immuno-reactive substance were in the granule of DNES( disperse or diffuse neuroendocrine system,DNES) cells. Lysozyme-immuno-reactive substance appeared in lymphoid tissue and increased when the body was infected with pathogen. This finding suggested that lysozyme-producing DNES cells might play an important role in intestinal mucosal immunity. It is an new proof for the neuro-endocrine-immune network theory.