RAPDs和RFLPs分析甘蓝型杂交油菜亲本的遗传多样性

Genetic Diversity of Parents for Hybrid Breeding in Brassica napus L. Detected by RAPDs and RFLPs

利用RAPD和RFLP分子标记技术对甘蓝型杂交油菜亲本遗传多样性的分析结果表明 :(1) 2 0个亲本间具有丰富的遗传多样性 ,4 0个引物扩增出 2 77条多态性带 ,其中 2 1条带为 10个亲本所特有 ,12个探针得到了 117条多态性杂交带 ,其中 7条带为 5个亲本所特有 ;(2 )不育系与恢复系之间的遗传差异大于不育系内和恢复系内的遗传差异 ;(3)依RAPDs与RFLPs估计的亲本遗传多样性存在有一定的差异 ,这种差异由估计时所用多态性带的数目不同而引起 ;(4)NJT聚类方法将 2 0份亲本分为 3组 :不育系组、恢复系组和混合组。杂交种“秦油 2号”和“华杂 3号”的双亲都被聚在不同的组内。

The genetic diversity of parents for hybrid breeding in Brassica napus L. were studied by RAPDs and RFLPs. The results showed that:(1) Large genetic variance was observed among 20 parents. Forty primers amplified 277 polymorphic bands, 21 of which were parent-specific. Twelve probes produced 117 polymorphic hybridized bands and 7 bands were parent-specific. (2) The genetic diversity among cytoplasm male sterile (CMS) lines and restorers was larger than that between male sterile lines or that between restorers. (3) Genetic diversity evaluated by RAPDs was different from that by RFLPs. The different polymorphic bands used to evaluate genetic diversity caused this difference. (4) Twenty parents were divided into three groups by NJT cluster analysis. One was the CMS group and another was restorer group. The last group was the mixture of CMS and restorer lines. Two parents of either the elite hybrid “Qinyou 2”or “Huaza 3”were clustered into different groups. The suitability of RAPDs and RFLPs in genetic diversity and their assistance to hybrids breeding were discussed.