海藻糖生产过程中产酶发酵条件的研究

Studies on enzyme fermentation condition by Micrococcus roseus during trehalose production

研究了产酶的培养基组分和比例以及最佳培养条件对微球菌生产麦芽寡糖基海藻糖合成酶 (MTSase)和麦芽寡糖基海藻糖海藻糖水解酶 (MTHase)的影响 ,得到最优培养基组成为 :葡萄糖 2 .0 % ,酵母膏 2 .0 % ,蛋白胨 1.0 % ,磷酸氢二钾 0 .1% ,硫酸镁 0 .0 5 % ;优化后的培养条件为 :以15 %的接种量接种至 2 5 0mL的锥形瓶中 ,装液量为 5 0mL ,初始 pH值 7.5～ 8.5 ,培养温度为30℃ ,摇床培养 4d。经优化后菌体干重由原来的 1.938g/L增加到 18.5 g/L ,生物量几乎增长了 10倍 ;而酶活也由原来的 30 .6 4U / g增加到 2 0 6 .11U / g ,酶活提高了接近 7倍。

The fermentation conditions including medium compositions and culture conditions by Micrococcus roseus were studied which affected biomass and enzymes activities on maltooligosyltrehalose synthase(MTSase) and maltooligosyltrehalose trehalohydrolase(MTHase) The result showed that the optimal medium contained glucose 2.0%, yeast extraction 2.0%, peptone 1.0%, K 2HPO 4·3H 20 0.1%, MgSO 4·7H 2O 0.05%. 15% spores and 50ml medium volumn were inculated in 250ml Erlenmeyer flask. And the optimal culture condition was initial pH 7.5～8.5, temperature 30℃, for 4 days.Under the optimized the medium and culture conditions, the biomass was increased from 1.938g/l to 18.5g/l and the enzyme activity enhance from 30.64U/g to 206.11U/g.