中华绒螯蟹卵巢新基因EJO6的全长cDNA克隆和序列分析

Cloning and Sequence Analysis of the Full-length cDNA of a Novel Gene: EJO6 from the Ovary of Eriocheir japonica sinensis

利用RACE技术从中华绒螯蟹卵巢获得了新基因EJO6(Eriocheirjaponicaovarygene6,EJO6)的全长cDNA序列(GenBank检索号:AY185922).该cDNA序列长度为1250bp,开放阅读框为690bp,编码229个氨基酸.根据氨基酸序列计算的相对分子质量和等电点分别为24270和11 9.同时用生物信息学的方法对该基因的结构和功能进行了初步分析.

In this report, a fulllength cDNA sequence of a novel gene named EJO6 from the ovary of Eriocheir japonica sinensis, which has been accepted by GenBank (Accession Number: AY185922), was obtained by RACE. The fulllength cDNA sequence of EJO6(Eriocheir japonica ovary gene 6, EJO6) is 1?250?bp in size and it has an open reading frame of 690?bp encoding a polypeptide of 229 amino acids. The pI/Mw deduced from amino acids sequence is 119 and 24?270 respectively. The function of EJO6 was preliminarily analyzed by means of bioinformatics.