摘要
利用RACE技术从中华绒螯蟹卵巢获得了新基因EJO6(Eriocheirjaponicaovarygene6,EJO6)的全长cDNA序列(GenBank检索号:AY185922).该cDNA序列长度为1250bp,开放阅读框为690bp,编码229个氨基酸.根据氨基酸序列计算的相对分子质量和等电点分别为24270和11 9.同时用生物信息学的方法对该基因的结构和功能进行了初步分析.
In this report, a fulllength cDNA sequence of a novel gene named EJO6 from the ovary of Eriocheir japonica sinensis, which has been accepted by GenBank (Accession Number: AY185922), was obtained by RACE. The fulllength cDNA sequence of EJO6(Eriocheir japonica ovary gene 6, EJO6) is 1?250?bp in size and it has an open reading frame of 690?bp encoding a polypeptide of 229 amino acids. The pI/Mw deduced from amino acids sequence is 119 and 24?270 respectively. The function of EJO6 was preliminarily analyzed by means of bioinformatics.
出处
《南京师大学报(自然科学版)》
CAS
CSCD
2003年第3期52-56,共5页
Journal of Nanjing Normal University(Natural Science Edition)
基金
教育部"211"工程建设项目.