摘要
探讨和优化红霉素肟及相关化合物的HPLC分析色谱条件 ,紫外检测波长为 2 0 5nm ,使用Hitachi色谱系统 ,ODS C1 8色谱柱 (1 5 0mm× 4mmI.D .) ,控制流速 1mL min ,流动相为磷酸二氢钾 乙腈 (65∶3 5 ) ,明确指认了红霉素肟合成中主要成分的Z、E异构体 ,红霉素 6,9 半缩酮 ,红霉素 6,9 9,1 2螺缩酮的位置 ,同时对红霉素A ,红霉素C的吸收峰位置也进行了辨析 ,线性相关性良好。对红霉素肟及相关化合物的详细HPLC解析 。
A high performance liquid chromatographic (HPLC) method for determination of erythromycin A oxime (EMAO), erythromycin, erythromycin A-6, 9-hemiketal, erythromycin A-6, 9-9 12-spiroketal and the main impurities is described. The chromatographic condition was optimized., An ODS 150 mm x 4 mm I. D. column was used and the Hitachi L-7420 ultraviolet detector was set at 205 nm; The composition of mobile phases is mixtures of 0.033 mol/L potassium phosphate-acetonitrile (65:35, V/V). The flow rate of mobile phase is I. 0 mL/min. The peak position of Z and E isomer of erythromycin oxime and corresponding substance are clearly identified. Linear relationship is good. It is very significant to understand the HPLC behavior of EMAO and other related substance's. and then supply us valuable information to improve the synthetic process.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2003年第9期1089-1092,共4页
Chinese Journal of Analytical Chemistry
基金
国家经贸委技术创新项目 (No .0 1BK 0 0 9)
