摘要
应用能量转移方法和光谱技术,研究了钙黄绿素-藏红T荧光能量转移体系与ctDNA的作用机理以及抑制作用的影响因素.在pH8.0和低离子强度条件下,ctDNA与Calcein-ST发生了静电电荷作用,降低了钙黄绿素-藏红T荧光体系的能量转移效率,对荧光体系的能量转移产生了明显的抑制作用.
Via spectrophotometric method and fluorescence transfer system, the reaction mechanism of Calcein- Safranine T with calf thymus DNA(ctGNA) was investigated. Factors on the inhibition of this fluorescence transfer system were also discussed. At pH 8.0 and low ionic strength, the static electric charge interaction of calf thymus DNA with Safranine T occurred and resulted in remarkable inhibition of the fluorescence energy transfer of Calcein- Safranine T pair.
出处
《分析测试技术与仪器》
CAS
2003年第3期147-151,共5页
Analysis and Testing Technology and Instruments
基金
国家教育部<高等学校骨干教师资助计划>
福建省科技攻关计划 (2 0 0 2Y0 51 )
福州大学科技发展基金 (2 0 0 2 -XQ -1 1 )等资助项目
