己二酸-N-羟基琥珀酰亚胺酯交联对胶原溶液流变性能的影响

Effects of N-hydroxysuccinimide Activated Adipic Acid Deriviate on the Rheological Behavior of Collagen Solution

为了促进胶原的开发和利用,使用流变仪考察了己二酸-N-羟基琥珀酰亚胺酯(NHS-AA)交联对胶原溶液稳态剪切、动态黏弹性、触变和蠕变的影响。稳态剪切实验表明,未交联的胶原溶液和NHS-AA交联后的胶原溶液均表现出明显的假塑性,其剪切黏度均随着剪切速率的增大而减小;同一剪切速率下,NHS-AA用量越大,胶原溶液的剪切黏度越大。通过动态黏弹性测试发现,随着NHS-AA的加入,胶原溶液的弹性模量显著增大,使得其弹性特征更明显。触变测试的结果表明,在胶原溶液中加入越多的NHS-AA,其触变环面积也越大,说明对胶原溶液施加剪切作用后,其结构更难回复了。蠕变测试结果显示,NHS-AA用量越大,胶原溶液柔量越小,意味着NHS-AA的加入可以增强胶原溶液抵抗形变的能力。以上实验结果证明NHS-AA交联可以调节胶原溶液的流变学性能。

For the development and utilization of collagen,the influences of N-hydroxysuccinimide activated adipic acid derivative(NHS-AA) on the steady flow,dynamic viscoelasticity,thixotropy and creep of collagen solution were studied by the rheometer.The results showed that the solutions cross-linked by different doses of NHS-AA were all pseudoplastic.The viscosity of collagen solutions increased with the increase of the dose of NHS-AA.The elastic modulus of collagen solutions increased dramatically due to the addition of NHS-AA and that indicated that NHS-AA reinforced the elasticity of collagen solution.The thixotropy of collagen solution became strong after adding NHS-AA,suggesting that the recovery of network structure broken by shear stress became slow.Adding NHS-AA resulted in the decrease of the compliance of collagen solution,reflecting that the cross-linked collagen solution was more difficult to transform with shear stress.All the experiments results indicated that the rheological properties of collagen solution could be adjusted by NHS-AA.