摘要
自从Liland C.Clark Jr.于1962年首次发明生物传感器以来,生物传感技术得到了突飞猛进的发展.目前,氧化锌纳米材料被视为极有前景的生物传感器构建材料之一,其具有多样化的纳米结构、高电子迁移率、化学稳定性、电化学活性、高等电点、生物相容性、压电特性等一系列出众的优异性能.本综述从电化学器件以及场效应器件两个角度介绍了氧化锌纳米材料在酶基生物传感器领域的应用.通过不同的合成工艺而获得的氧化锌纳米结构被用于酶分子的装载与固定,并同时为酶提供一个良好的微环境,从而有效提升了生物传感性能.本文综述了氧化锌纳米材料合成工艺的最新进展,针对不同的氧化锌纳米结构对传感器的性能进行了对比,并总结了氧化锌纳米材料在生物传感器构建中的主要优势,以及未来的发展前景和挑战.
Biosensing has developed tremendously since it was demonstrated by Leland C. Clark Jr. in 1962. ZnO nanomaterials are attractive candidates for fabricating biosensors,because of their diverse range of nanostructures,high electron mobility,chemical stability,electrochemical activity,high isoelectric points which promote enzyme adsorption,biocompatibility,and piezoelectric properties. This review covers Zn O nanostructures applied in enzyme biosensors,in the light of electrochemical transduction and field effect transduction. Different assembly processes and immobilization methods have been used to load enzymes into various ZnO nanostructures,providing enzymes with favorable micro-environments and enhancing their sensing performance. We briefly describe recent trends in ZnO syntheses,and the analytical performance of the fabricated biosensors,summarize the advantages of using ZnO nanostructures in biosensors,and conclude with future challenges and prospects.
基金
supported by the National Basic Research Program of China (2013CB932600)
the Major Project of International Cooperation and Exchanges (2012DFA50990)
the Program of Introducing Talents of Discipline to Universities
the National Natural Science Foundation of China (51232001,51172022 and 51372023)
the Research Fund of Co-construction Program from Beijing Municipal Commission of Education
the Fundamental Research Funds for the Central Universities
the Program for Changjiang Scholars and Innovative Research Team in University
