摘要
荧光生物分析技术在科研及医疗机构已获得广泛应用.常规的荧光免疫分析方法由于采用传统生物探针(如荧光染料及量子点等)作为标记,易受到杂散光及生物组织自荧光的干扰.利用无机稀土纳米荧光探针优异的发光性能,如长荧光寿命的下转移发光、近红外激发的上转换发光以及无需激发源的长余辉发光,可有效解决背景荧光的干扰.本文从基础的物理化学性能到生物应用角度出发综述了无机稀土纳米发光材料的最新进展,包括材料的控制合成、表面功能化、光学性能及其在无背景荧光生物分析方面的应用示范,并对该类材料未来的发展趋势与努力的方向作了进一步的远景展望.
Luminescent bioassay techniques have been widely adopted in a variety of research and medical institutions. However, conventional luminescent bioassays utilizing traditional bioprobes like organic dyes and quantum dots often suffer from the interference of background noise from scattered lights and autofluorescence from biological matrices. To eliminate this disadvantage, the use of inorganic lanthanide(Ln3+)-doped nanoparticles(NPs) is an excellent option in view of their superior optical properties, such as the long-lived downshifting luminescence, near-infrared triggered anti-Stokes upconverting luminescence and excitation-free persistent luminescence. In this review, we summarize the latest advances in the development of inorganic Ln3+-doped NPs as sensitive luminescent bioprobes from their fundamental physicochemical properties to biodetection, including the chemical synthesis, surface functionalization, optical properties and their promising applications for background-free luminescent bioassays. Future efforts and prospects towards this rapidly growing field are also proposed.
基金
supported by the National Basic Research Program of China (2014CB845605)
Special Project of National Major Scientific Equipment Development of China (2012YQ120060)
the National Natural Science Foundation of China (11204302, 11304314, U1305244, U1405229 and 21325104)
the Chinese Academy of Sciences (CAS)/State Administration of Foreign Expert Affairs International Partnership Program for Creative Research Teams
the CAS CrossDisciplinary & Collaborative Research Team Program
the Strategic Priority Research Program and Scientific Equipment Development Project of the CAS (XDA09030307 and YZ201210)
the Key Project of Science and Technology of Fujian Province (2013H0060)
