摘要
We report a simple approach for the fabrication of cheap but effective surface-enhanced Raman scattering(SERS) active substrates consisting of natural egg shell membrane(ESM) grafted with Ag-nanoparticles(Ag-NPs) decorated carbon nanotubes(CNTs)(denoted as Ag-NPs@CNTs@ESM),via ultrasonic adsorption of CNTs on the ESM and the subsequent Ag-sputtering.As high density Ag-NPs were sputtered onto each CNT anchored on the ESM nanofiber,homogeneous nanoscaled gaps between the neighboring Ag-NPs were achieved,leading to high SERS activity with excellent SERS signal homogeneity over the whole membrane.Using the Ag-NPs@CNTs@ESM membranes as SERS-substrates,methyl parathion(one of the most hazardous insecticides),PCB-3(one congener of polychlorinated biphenyls belonging to persistent organic pollutants) and bovine serum albumin(BSA,biomolecules) with a concentration down to 10-11 M,10-6 M and 0.1 ppb were detected respectively,showing promising potentials in rapid trace detection of environmental pollutants and biochemicals.
本文报道了一种利用具有均匀纤维网络结构的鸡蛋膜作为三维骨架,制备碳纳米管/鸡蛋膜复合表面增强Raman散射(SERS)衬底的简单廉价的方法.首先,借助超声吸附,将碳纳米管组装到经过前处理的鸡蛋膜纤维的表面,得到均匀的碳纳米管/鸡蛋膜复合膜;然后,借助溅射方法,将银纳米颗粒组装到碳纳米管和鸡蛋膜纤维的表面,得到银纳米颗粒修饰的碳纳米管/鸡蛋膜复合膜.这种方法制备的复合膜表面的碳纳米管对小分子目标分析物有很好的吸附和富集作用;同时,由于高密度银纳米颗粒的存在,衬底表面有大量的SERS活性位点,因此这种复合膜是一种有效的SERS衬底.研究表明,采用这种银纳米颗粒修饰的碳纳米管/鸡蛋膜复合SERS衬底,可实现对农药甲基对硫磷、多氯联苯以及生物分子牛血清蛋白等的快速痕量识别.由于该衬底的制备方法简单、原材料环保且来源广泛、衬底的活性高,所以该衬底在基于SERS技术对环境污染物和生物分子的快速痕量检测方面具有广泛的应用前景.
基金
supported by the National Key Basic Research Program of China(2013CB934304)
the National Natural Science Foundation of China(21307138,11274312,11175204 and 51472245)
the State Administration of Foreign Experts Affairs of Chinese Academy International Partnership Program for Creative Research Teams
the President Foundation of Hefei Institutes of Physical Science,Chinese Academy of Sciences(YZJJ201312)
