Multiplexed micro RNA TG-FRET assay with isothermal and amplification-free single-step

Human genome encodes more than 2000 distinct micro RNAs(Micro RNA database:http://www.mirbase.org/),a class of single-strand noncoding RNAs with a short length of 19 to 25 nucleotides[1].They take part as regulatory factors in almost all biological processes from cell proliferation,differentiation to death.Since the alternation in the micro RNA expression level can be associated with a num-