摘要
本文利用一种叶酸代谢产物,对氨基苯甲酸(PABA),来改性碳纤维(CFs),通过在其表面形成微槽结构以达到改善其亲水性的目的.采用溶液浇注/粒子沥滤法制备了CFs/PLA-PEG复合生物支架.研究发现,引入小的PABA疏水分子可以在CFs和聚乳酸-聚乙二醇(PLA-PEG)基体之间形成良性结合界面.CFs/PLA-PEG生物支架的抗压强度从3.98MPa提高到5.48MPa.由于PABA的改性,低毒性的CFs/PLA-PEG复合材料显著加速了预成骨细胞的粘附,对细胞增殖没有明显影响.通过比较支架上的L929细胞和MC_3T_3细胞的生物相容性,可以认为PABA-CFs不仅克服了PLA-PEG强度差的缺陷,而且可以促进细胞的生长,证明了PABA-CFs增强PLA-PEG生物复合材料可作为一种潜在的组织工程支架材料.
Para-amino benzoic acid(PABA),a folic acid related metabolite,was first introduced to fabricate micro-grooves and improve hydrophilicity over surfaces of carbon fibers(CFs).Then,engineered CFs/poly(lactic acid)-poly(ethylene glycol)(PLA-PEG) biocomposites were fabricated by a solvent casting/particulate leaching method.We found that introducing small hydrophobic PABA molecules and fabricating patterned structures would lead to benign integrated interfaces between CFs and the PLA-PEG matrix.Specifically,the compressive strength of CFs/PLA-PEG was improved from 3.98 to 5.48 MPa.In addition,the CFs/PLA-PEG biocomposites significantly accelerated the adhesion and proliferation of pre-osteoblasts with minimized cytotoxidty.By comparing the cyto-compatibility of L929 and MC_3T_3 cells cultured on different modified PLA-PEG composites,it could be concluded that PABA-CFs not only overcame the limitation of poor strength of PLA-PEG,but also improved the cell growth.These results indicate that the PABA-CFs reinforced PLA-PEG biocomposites could be a potential alternative for tissue engineering scaffolds.
基金
supported by the National Key Research and Development Project(2016YFB0303201)
the Research and Innovation Project of Shanghai Municipal Education Commission(14zz069)
Donghua University Graduates'Innovation Funding Projects(EG2015006)
