摘要
随着对大量生物分子的高通量分析的需求不断增长,多元分析成为用于进行大规模生物分析的前景技术.在目前的技术中,基于编码微载体的液相芯片已被广泛用于如临床、医学、营养和环境等诸多研究领域的多元生物检测中.这些编码微载体除了具有独特的编码形式,还具有更高的灵活性,更好的灵敏度及更快的反应动力学.在本综述中,我们根据编码方法描述了一些常见的编码微载体,并且介绍了它们在不同靶标(如蛋白质,核酸及细胞)的多元生物分析中的应用,以及在不同领域(如监测食品安全,药物研究和临床诊断)中的应用.
With the increasing demand for multiplex and high-throughput analysis of large numbers of biomolecules,multiplex technology becomes a promising tool for carrying out thousands of individual reactions at the same time for large-scale biological analysis. Among current technologies,suspension arrays based on appropriate barcode particles have been popularly used in multiplex bioassays of many research fields with the ability of unique encoding, such as in the clinical, medicinal, nutritional, and environmental fields.Besides the unique form of barcode, these particles have higher flexibility, better sensitivity, and faster reaction kinetics. In this review, we present some examples of typical barcode particles that are divided into different groups depending on how they are encoded and their applications in multiplex bioassays for different targets such as proteins,DNA and RNA sequences, and cells. The bioassays for monitoring food safety, drug research, and clinical diagnosis are also described.
作者
徐月霜
王欢
陈宝安
刘宏
赵远锦
Yueshuang Xu;Huan Wang;Baoan Chen;Hong Liu;Yuanjin Zhao(Department of Hematology and Oncology,Zhongda Hospital,School of Medicine,Southeast University,Nanjing 210009,China;State Key Laboratory of Bioelectronics,School of Biological Science and Medical Engineering,Southeast University,Nanjing 210096,China)
基金
supported by the National Natural Science Foundation of China (21473029 and 51522302)
the NSAF Foundation of China (U1530260)
the Key Medical Projects of Jiangsu Province ( BL2014078)
Key Discipline of Jiangsu Province (2016-2020)
the Natural Science Foundation of Jiangsu (BK20140028)
the Scientific Research Foundation of Southeast University
