维生素C对普安银鲫胚胎发育中两种脂酶活性的影响

Activities of Two Lipases and Vitamin C Effects during Embryonic Development in Crucian Carp Carassius auratus

在水温24℃下,研究了普安银鲫(Carassius auratus)胚胎发育过程中脂蛋白脂酶(LPL)、肝脂酶(HL)和总脂酶(GE)活性的变化特点及维生素C溶液浸泡对其影响。结果显示:(1)胚胎中LPL、HL和GE的比活力与全活力比成熟卵中高。(2)随胚胎发育,LPL、HL和GE比活力与全活力上升,自原肠中期始,LPL和HL的活性显著升高(P<0.05),出膜前期达最高。(3)30mg/L维生素C溶液浸泡促使普安银鲫胚胎发育过程中LPL、HL和GE比活力与全活力升高。合子中LPL和HL基因可能在原肠期开始表达,合成脂质水解酶分解脂质,为胚胎发育提供能量。在水体中添加适宜水平的维生素C能促进普安银鲫胚胎发育,维持机体内脂质的正常代谢。

The activities of lipoprotein lipase, hepatic lipase and general lipid enzyme and vitamin C effects were studied at water tem-perature of 24℃during embryonic development of crucian carp Carassius auratus by biochemical methods. The results showed that there were higher activities of lipoprotein lipase, hepatic lipase and general lipid enzyme in embryos were than those in mature eggs. During the embryonic development,the specific activity and total activity were found to be increased with embryonic development, significant increase from mid-gastrula stage, and the maximal activities of two lipase enzyme at prehatching. Vitamin C solution at a dose of 30mg/L led to increase in the activities of lipoprotein lipase, hepatic lipase and general lipid enzyme during embryo develop-ment. The findings indicated that lipoprotein lipase and hepatic lipase genes in zygotes may start to play function at gastrula stage when lipid hydrolytic enzymes are synthesized to release energy for embryonic development. The appropriate dose of vitamin C in water could play an important role in maintaining normal lipid metabolism in body, thus promoting embryonic development of the crucian carp.