褪黑素对小鼠卵母细胞体外自发成熟的影响

Effect of Melatonin on Oocyte Spontaneous Maturation of Mouse in Vitro

本文通过卵母细胞自发成熟体外培养模型研究了褪黑素(MT)对小鼠如母细胞成熟的影响。结果表明:0.1mg/ml、0.02mg/ml、0.004mg/mg及0.0008mg/ml浓度的MT均能显著抑制小鼠卵丘卵母细胞复合体(CEO_s)自发成熟过程(P<0.01)。但对裸卵(DO_s)自发成熟,没有影响。结论:MT是调节哺乳动物卵母细胞成熟的重要激素之一,其作用机制可能是通过卵丘细胞实现的。

Mouse oocytes were cultured in maturation medium, and the effects of MT on meiotic maturation of mouse oocyte were examined. The results show MT at all doses of 0. 1mg/ml、0. 02mg/ml、0. 004mg/ml or 0. 0008mg/ml inhibited the formation of PB1 in CEOs cultured in maturation medium and ha no effect on GVBD.but not in DOs. Conclusion: MT is able to affect mouse oocyte maturation and the regulation mechanisms may be related to cumulus cells.