中西药治疗糖尿病早期大鼠视网膜病的机制

Mechanism of Treatment of Tetramethylprazine and Aminoguanidine for Diabetic Retinopathy in the Early Diabetic Rats

目的通过观察中药川芎嗪联合西药氨基胍治疗对糖尿病早期大鼠视网膜组织醛糖还原酶(aldose reductase,AR)和一氧化氮合酶(nitric oxide synthase,NOS)含量的影响,探讨中西药治疗糖尿病视网膜病(dirabetic retinopathy,DR)的治疗机制。方法选择健康成年雄性SD大鼠,随机分成正常对照组、糖尿病组、糖尿病川芎嗪治疗组、糖尿病氨基胍治疗组和糖尿病川芎嗪联合氨基胍治疗组。一次性腹腔注射链脲佐菌素(streptozotocin,STZ)诱发糖尿病模型,于第90d测定各组大鼠视网膜组织AR和NOS含量。结果糖尿病川芎嗪治疗组、糖尿病氨基胍治疗组和糖尿病川芎嗪联合氨基胍治疗组的大鼠视网膜组织AR和NOS含量低于糖尿病组、糖尿病川芎嗪联合氨基胍治疗组的大鼠视网膜组织AR和NOS含量低于糖尿病川芎嗪治疗组和糖尿病氨基胍治疗组。结论结合以往观察的川芎嗪联合氨基胍治疗对大鼠眼底和视网膜组织学的影响结果,川芎嗪和氨基胍可通过降低糖尿病早期大鼠视网膜AR和NOS含量而防治DR,联合用药效果更佳。

Purpose To investigate the protective effect of tetramethylprazing(TMP)and aminoguanidine ( AG) on diabetic retinopathy and explore its possible mechanism by determining the contents of aldoes reductase( AR)and nitric oxide synthase(NOS)in the retina of early diabetic rats. Methods The contents of AR and NOS in the retina of the streptozotocin-induced diabetic rats were determined on the 90 th day. Results After 90 days the contents of AR and NOS in the retina of diabetic rats were increased compared with other rats, the contents of AR and NOS in the retina of diabetic rats treated with TMP or AG were decreased compared with diabetic rats, and the contents of AR and NOS in the retina of diabetic rats treated with TMP combined with AG were decreased compared with diabetic treated with TMP or AG. Conclusion TMP combined with AG may prevent the development of diabetic retinopathy by reducing the contents of AR and NOS in the retina of early diabetic rats.