多囊卵巢综合征患者颗粒细胞叉头框蛋白O1 mRNA的表达与意义

Expression of Forkhead box protein O1 mRNA in granulosa cells of polycystic ovary sydrome patients and its significance

目的探讨转录因子叉头框蛋白O1(FOXO1)在多囊卵巢综合征(PCOS)患者卵巢颗粒细胞中的表达及意义。方法采用逆转录实时定量PCR(qRT-PCR)检测67例PCOS患者(PCOS组)与63例对照患者(对照组)卵巢颗粒细胞中FOXO1的表达,并分析其临床意义。结果PCOS组的体质量指数(BMI)、胰岛素抵抗指数(HOMA-IR)、黄体生成素(LH)、睾酮(T)、抗苗勒氏管激素(AMH)、卵巢基础卵泡数(AFC)明显高于对照组(P均<0. 05),但PCOS组的卵泡刺激素(FSH)低于对照组(P <0. 05)。与对照患者相比,FOXO1在PCOS患者卵巢颗粒细胞中的表达下降(P <0. 05)。采用二元Logistic回归分析发现,FOXO1、AFC、HOMA-IR是影响PCOS的危险因素(P <0. 05)。结论 FOXO1在PCOS患者卵巢颗粒细胞低表达,可能在PCOS的发生发展中发挥重要作用。

Objective To explore the expression and significance of Forkhead box protein O1(FOXO1)in ovarian granulosa cells of patients suffering from polycystic ovary syndrome(PCOS).Methods A total of 130 patients,including 67 PCOS patients and 63 controls,were enrolled.The expression of FOXO1 in ovarian granulosa cells was examined by realtime quantitative reverse transcription PCR(qRT-PCR),and the clinical significance was analyzed.Results PCOS patients had significantly higher body mass index(BMI),homeostasis model assessment-insulin resistance(HOMA-IR),luteotropic hormone(LH),testosterone(T),anti-Mullerian hormone(AMH),average follicle count(AFC)levels(all P<0.05),but lower FSH level(P<0.05)than controls.Compared with controls,the expression of FOXO1 was much lower in granulosa cells of PCOS patients(P<0.05).Binary Logistic regression analysis revealed that FOXO1,AFC and HOMA-IR were risk factors influencing the occurrence of PCOS(P<0.05).Conclusion The lower expression of FOXO1 in the granulosa cells of PCOS patients may play an important role in the occurrence and development of PCOS.