酸浆苦素B对人非小细胞肺癌细胞增殖、迁移及凋亡的影响

Effects of Physalin B on proliferation,migration and apoptosis of human non-small cell lung cancer cells

目的探讨酸浆苦素B(PB)对非小细胞肺癌(NSCLC)A549细胞增殖、迁移和凋亡的影响,初步探讨其作用机制。方法细胞分为不同浓度(2.5、5、10、20、40μmol/L)PB组、对照组、空白组,实时无标记细胞分析技术(RTCA)、CCK-8法检测PB对A549细胞增殖抑制率变化,Transwell检测细胞迁移,流式细胞仪检测PB对A549细胞的凋亡及周期的影响,Western blotting检测PB对相关蛋白表达的影响。结果 2.5、5、10、20、40μmol/L PB可抑制A549细胞增殖,且呈剂量依赖性;Transwell结果显示,10、20μmol/L PB组较对照组的细胞迁移能力下降;10、20μmol/L PB处理后,A549细胞的凋亡率升高,呈剂量依赖性。与对照组相比,10、20μmol/L PB组G_2/M期细胞比例增高、G_0/G_1期比例减少。Western blotting检测结果显示,与对照组相比,10、20μmol/L PB组使Akt蛋白表达水平下降,而Caspase-9和PTEN蛋白表达水平上升。结论 PB可抑制A549细胞的增殖,诱导细胞凋亡且细胞周期阻滞,这可能与调控细胞周期相关蛋白表达有关。

Objective To investigate the effects of Physalin B(PB)on proliferation,migration and apoptosis of A549cells in non-small cell lung cancer(NSCLC),and preliminarily explore its mechanism.Methods The A549 cells w ere divided into different concentrations(2.5,5,10,20,40μmol/L)of PB groups,control group and blank group.The inhibition rate of PB on A549 cells proliferation w as detected by real-time cell analyzer(RTCA)and CCK-8 assay,cell migration w as examined by transw ell chamber assay,the apoptosis and cycle of PB on A549 cells w ere detected by flow cytometry and the effect of PB on the expression of related proteins w as detected by Western blotting.Results PB w ith2.5,5,10,20 and 40μmol/L significantly inhibited the proliferation of A549 cells in a dose-dependent manner.The cell migration ability of 10 and 20μmol/L PB groups w as significantly low er than that of control group.After treatment w ith 10 and 20μmol/L PB,the apoptosis rate of A549 cells increased in a dose-dependent manner.Compared w ith control group,the proportion of G2/M phase cells increased and the proportion of G0/G1phase cells decreased in 10 and 20μmol/L PB groups.Compared with the control group,the expression level of Akt protein decreased while the expression levels of Caspase-9 and PTEN increased in 10 and 20μmol/L PB groups.Conclusion PB can inhibit the proliferation of A549 cells,induce cell apoptosis and cell cycle arrest,w hich may be related to the regulation of cell cycle related protein expression.