袖状胃切除术对2型糖尿病大鼠肺组织损伤的影响

Effects of sleeve gastrectomy on lung injury in type 2 diabetic rats

目的探讨袖状胃切除术对2型糖尿病大鼠肺组织损伤的影响及其机制。方法 Wistar大鼠腹腔内注射链脲霉素诱导2型糖尿病大鼠模型,将2型糖尿病大鼠随机分为两组:袖状胃切除组(SG组,n=10)及假手术组(Sham组,n=10)。术后观察大鼠体质量、进食量、空腹血糖(FBG),并行口服糖耐量试验(OGTT)。术后第16周处死大鼠,取大鼠肺组织,行HE染色观察肺组织形态学表现;采用Western blotting以及免疫组化技术检测肺组织内炎症因子白介素6(IL-6)、肿瘤坏死因子α(TNF-α)及氧化应激指标硝基酪氨酸(NT)的表达;生化检测肺组织内氧化应激指标一氧化氮(NO)、丙二醛(MDA)的表达及超氧化物歧化酶(SOD)的活力。结果 SG组大鼠术后死亡1只,其他大鼠无死亡。SG组大鼠相较于Sham组大鼠血糖下降(FBG:F=100.70,P<0.01;OGTT曲线下面积AUC_(OGTT):F=88.15,P<0.01),肺组织肺泡间隔增厚情况减轻、肺泡内出血减少, IL-6、TNF-α、NT、NO、MDA的表达减少(P<0.01),SOD的活力增加(P<0.01)。结论袖状胃切除术可以缓解2型糖尿病大鼠的肺组织损伤,可能是通过控制血糖、进而减少肺组织内炎症因子的表达和改善肺组织内氧化应激状态来实现。

Objective To investigate the effects of sleeve gastrectomy on lung injury in type 2 diabetic rats and to explore the mechanism.Methods Type 2 diabetic models were induced by intraperitoneally injecting streptozotocin(STZ)to Wistar rats.The diabetic rats were randomly assigned to the sleeve gastrectomy group(SG group,n=10)and Sham group(n=10).The body weight,food intake,fasting blood glucose(FBG)and glucose tolerance were measured postoperatively.In postoperative week 16,the rats were sacrificed.The lung tissues were collected and the histomorphology was observed with HE staining.The expressions of inflammation factors interleukin-6(IL-6),tumor necrosis factorα(TNF-α)and oxidative stress indicator nitrotyrosine(NT)were determined with Western blotting and immunohistochemistry.The expressions of nitric oxide(NO)and malondialdehyde(MDA),as well as the activity of superoxide dismutase(SOD)were determined with biochemical testing.Results Only 1 rat in the SG group died after surgery.Compared with the sham group,SG group had better glucose control(FBG:F=100.70,P<0.01,AUCOGTT:F=88.15,P<0.01),mitigated thickness of interalveolar septum,reduced hemorrhage,lower expressions of IL-6,TNF-α,NT,NO and MDA(P<0.01),but higher activity of SOD(P<0.01).Conclusion Sleeve gastrectomy can relieve the lung injury in type 2 diabetic rats,possibly by inhibiting the expressions of inflammation factors and improving oxidative stress in lungs.