去腹壁运动神经支配建立兔腹壁薄弱模型

To establish abdominal wall weakness model in denervated rabbits

目的通过采用直接离断和离断并切除的方式切断实验兔的一支或相邻多支腹壁运动神经,建立实验兔去腹壁运动神经支配导致腹壁薄弱的动物模型。方法将20只实验兔采用数字表法分为4组,通过外科操作损毁一侧腹壁的运动神经,第1组离断1支腹壁运动神经,第2组离断相邻的3支腹壁运动神经,第3组离断并切除1 cm长度的1支腹壁运动神经,第4组离断并切除1 cm长度的相邻3支腹壁运动神经,采集外科操作后实验兔两侧的腹围数据,通过对比两侧腹围情况观察实验兔外科操作后腹壁薄弱的发生情况,以验证腹壁薄弱动物模型的建立。结果 20例实验兔术后伤口均愈合,1只术后出现局部红肿,2周后消退;第1组、第2组和第3组实验兔两侧腹围未见明显变化,无腹壁薄弱,第4组5只实验兔操作侧腹围明显增加,出现腹壁薄弱,证实该组腹壁薄弱模型制备成功。结论以离断并切除相邻多支腹壁运动神经的方法制备兔腹壁薄弱模型成功,该方法操作简单,效果良好,为腹壁薄弱的研究提供了可靠的动物模型。

Objective To establish abdominal wall weakness model by motor nerve denervation in experimental rabbits.Methods Twenty experimental rabbits was randomly divided into 4 groups,one or multiple adjoining abdominal wall motor nerves were destroyed by cut off or excision of 1 cm on ventral wall of rabbits:one nerve was cut off in group 1,three adjacent nerves were cut off in group 2,one nerve was excised 1 cm in group 3,three adjacent abdominal wall nerves were excised 1 cm in group 4.The length of bilateral abdominal wall was recorded and compared to evaluate the abdominal wall weakness and to verify the establishment of animal model.Results All experimental rabbits survived after operation,one operation wound was inflamed and recovered after 2 weeks,the length of bilatera abdominal wall had no significant change in group 1,group 2 and group 3,however,the length of abdominal wall after operation was markedly increased compared with the other side in group 4,the abdominal wall weakness appeared and the animal model was established successfully in group 4.Conclusion We successfully established the animal model of abdominal wall weakness in experimental rabbits by excising multiple adjoining abdominal wallnerves.This method is simple and feasible.It is a reliable animal model for the study of abdominal wall weakness.