摘要
目的采用长期低剂量皮下注射鱼藤酮的方法制备SD大鼠帕金森病模型。方法将36只SD大鼠随机分为实验组24只和对照组12只,实验组给予l.5 mg/(kg·d)鱼藤酮皮下注射,对照组注射等体积葵花油,连续30 d,每周停药1 d。给药结束后进行行为学测试,并断头取脑,采用免疫组化SP法检测中脑黑质多巴胺能神经元数目。结果对照组行为学改变不明显,其黑质多巴胺能神经元数目较多;实验组出现明显的行为学改变,其黑质多巴胺能神经元数目减少,符合PD的诊断标准。结论颈背部皮下注射鱼藤酮可成功制备SD大鼠帕金森病模型。
Objective To established PD models by long term subcutaneous injection of rotenone. Methods Totally36 SD rats were randomly divided into two groups: control group contained 12 rats and model building group contained 24 rats. Rats in model building group were treated with rotenone at dose of 1. 5 mg /( kg·d) by subcutaneous injection for 30 days,withdrawal one day a week. Rats in control group were treated with the same volume of sunflower oil. After the end of subcutaneous injection,the behavior changes of SD rats were tested and then sacrificing the rats to take out the brain tissues,detecting the expression of TH-positive cells in substantia nigra by counting out the number of TH-positive cells. Results The behavior changes of SD rats in control group were not obvious,but the number of dopaminergic neurons increased. The behavior changes of SD rats in model building group was obvious,and their dopaminergic neurons numbers decreased. SD rats in model building group met the diagnostic criteria of PD. Conclusions The methods of long-term subcutaneous injection of rotenone could establish PD models in SD rats.
出处
《山东医药》
CAS
北大核心
2015年第1期9-11,14,共4页
Shandong Medical Journal
基金
广东省科技计划项目(2010B060500015)
广东省科技计划项目(2013B060300038)
