丙泊酚对脑缺血再灌注大鼠脑组织Homer-1a表达的影响及其机制

Effect of propofol on expression of Homer-1a in cerebral ischemia reperfusion rats and the mechanism

目的观察丙泊酚对脑缺血再灌注大鼠脑组织Homer-1a表达的影响,并探讨其机制。方法雄性SD大鼠120只,随机分为假手术组、造模组、丙泊酚组,每组40只。模型组、丙泊酚组线栓法建立脑缺血再灌注模型,假手术组仅行血管分离处理不插入线栓;造模后30 min,丙泊酚组腹腔注射丙泊酚10 mg/100 g,假手术组和对照组均腹腔注射等体积生理盐水。给药处理3 h、24 h、72 h、7 d分别进行神经功能评分,采用Real-time PCR法检测脑组织Homer-1a mRNA,应用酶联免疫吸附法检测血清S-100β。结果与假手术组比较,模型组和丙泊酚组4个时间点神经功能评分、血清S-100β水平均增加(P均<0.05),以模型组增高更显著(P均<0.05)。与假手术组相比,模型组在3、24 h时间点脑组织Homer-1a mRNA降低(P均<0.05);丙泊酚组4个时间点脑组织Homer-1a mRNA均较其他两组升高(P均<0.05)。在所有大鼠样本中,脑组织Homer-1a mRNA表达与血清S-100β呈负相关(r=-0.939,P<0.01)。结论丙泊酚麻醉可通过诱导脑组织Homer-1a mRNA表达发挥对脑缺血再灌注大鼠的脑保护作用,该作用可能与其降低血清S-100β水平有关。

Objective To observe the effect of propofol on the expression of Homer-1a in the brain tissues of cerebral ischemia reperfusion rats,and to explore its mechanism. Methods A total of 120 male SD rats were randomly divided into the sham operation group,model group and propofol group,40 rats in each group. Cerebral ischemia reperfusion rat models were established in the model group and propofol group by using the suture method; sham operation group only received vascular separation processing,without line bolt insertion. Thirty min after modeling,rats in the propofol group were given propofol 10 mg /100 g by intraperitoneal injection,while rats in the sham operation and control groups were injected the same amount of normal saline. After the drug treatment for 3 h,24 h,72 h and 7 d,neurological function score was evaluated,Homer-1a mRNA level in the brain tissues was detected by using the real-time PCR,and S-100β in the peripheral blood was detected by enzyme linked immunosorbent assay. Results Compared with the sham operation group,the neurological function scores and S-100β levels of peripheral blood were increase at the four time points in the model and propofol groups( all P < 0. 05),and the model group increased more significantly( all P < 0. 05). Compared with the sham operation group,the level of Homer-1a mRNA decreased in the model group at the time points of 3 h and 24 h( all P < 0. 05).The Homer-1a mRNA level of the propofol group was higher than those in the other two groups at the four time points( all P < 0. 05). In all rat samples,Homer-1a mRNA expression level and S-100β level was negatively correlated( r =- 0. 939,P < 0. 01). Conclusion Propofol anesthesia has a protective effect on the brain tissues in the cerebral ischemia reperfusion rats by inducing Homer-1a mRNA expression,and this effect may be related to the decrease of S-100β level in the peripheral blood.