外阴鳞状上皮增生皮损组织中K17、IFN-γ、PCNA的表达变化及意义

Expression and significance of K17,IFN-γ and PCNA in skin lesions of patients with squamous hyperplasia of vulva

目的观察外阴鳞状上皮增生皮损组织中角蛋白17(K17)、干扰素γ(IFN-γ)、增殖细胞核抗原(PCNA)的表达变化,并探讨其意义。方法外阴鳞状上皮增生患者27例,取其皮损组织作为A组,取病灶周围正常皮肤组织作为B组。采用免疫组化SP法检测两组PCNA、K17和IFN-γ的表达,并分析其相关性。结果 A组增殖指数26.24%±6.60%,B组10.47%±4.77%,P<0.01。A组K17、IFN-γ的光密度值均高于B组(P均<0.01)。B组K17与IFN-γ的表达无相关性(r=0.122,P>0.05),A组K17与IFN-γ表达呈正相关(r=0.607,P<0.01)。结论外阴鳞状上皮增生可能与皮损组织中PCNA、K17、IFN-γ表达增加有关。

Objective To observe the expression levels of the keratin 17( K17),interferon-γ( IFN-γ) and proliferating cell nuclear antigen( PCNA) in skin lesions of patients with squamous hyperplasia of vulva,and to explore their significance. Methods Twenty-seven patients with squamous hyperplasia of vulva,the lesions were taken as group A and the normal epidermis surrounding the lesions as the group B. The expression levels of PCNA,K17 and IFN-γ were detected by the immunohistochemical SP method, and the correlations with the squamous hyperplasia of vulva were analyzed.Results The proliferation indexes of group A and group B were 26. 24% ± 6. 60% and 10. 47% ± 4. 77%,respectively( P < 0. 01). The average optical density of K17 and IFN-γ in the group A was significantly higher than that in the group B( all P < 0. 01). In the group B,the expression of K17 was not related with the IFN-γ expression( r = 0. 122,P >0. 05). In the group A,the expression of K17 was positively correlated with the IFN-γ expression( r = 0. 607,P < 0. 01).Conclusion The increased expression levels of K17,IFN-γ and PNCA in the skin lesions of patients with squamous hyperplasia of vulva has significantly relevance to its pathogenesis.