Anti-CD_(20)与IL-10联合应用对非肥胖型糖尿病小鼠脾脏免疫功能的影响

Influence of anti-CD_(20) monoclonal antibody combined with IL-10 on immune function of spleen in NOD mice

目的观察抗CD20单克隆抗体(Anti-CD20)与白细胞介素10(IL-10)联合应用对非肥胖型糖尿病(NOD)小鼠脾脏免疫功能的影响。方法将24只NOD小鼠随机分为A、B、C、D组各6只,分别于第1、8、15、21天尾静脉注射Anti-CD20250μg、Anti-CD20250μg+IL-10 0.1 m L、IL-10 0.1 m L和生理盐水0.1 m L。首次用药后第12周断颈处死小鼠,立即取出脾脏,采用免疫组化法检测脾脏组织中的CD+4、CD+3T细胞及IL-27、IL-10。结果与D组比较,A、B、C组脾脏组织中CD+4、CD+3T细胞数量及IL-27、IL-10表达增加(P均<0.01);与A、C组比较,B组脾脏组织中CD+4、CD+3T细胞数量及IL-27、IL-10表达增加(P均<0.01)。结论 Anti-CD20与IL-10联合应用可增加NOD小鼠脾脏组织中CD+4、CD+3T细胞数量及IL-27、IL-10表达,从而调节机体免疫微环境,预防糖尿病的发生。

Objective To observe the influence of anti-CD20 monoclonal antibody( Anti-CD20) combined with interleukin 10( IL-10) on the immune function of spleen in non-obese diabetic( NOD) mouse. Methods Twenty-four NOD mice were randomly divided into four groups,6 mice in each group: groups A,B,C and D,which were caudal venously injected with 250 μg Anti-CD20,250 μg Anti-CD20+ 0. 1 m L IL-10,0. 1 m L IL-10 and 0. 1 m L normal saline at day 1,8,15 and 21,respectively. All mice were sacrificed 12 weeks after first injection and the spleens were immediately taken out.The number of CD+4T cells,CD+3T cells and the levels of IL-10 and IL-27 were detected by immunohistochemistry.Results Compared with group D,the number of CD+4T cells,CD+3T cells and the levels of IL-10 and IL-27 in the spleen tissues were increased in the groups A,B and C( all P < 0. 01). Compared with groups A and C,the number of CD+4T cells,CD+3T cells and the levels of IL-10 and IL-27 in the spleen tissues were increased in the group B( all P < 0. 01).Conclusion The combined administration of Anti-CD20 and IL-10 increases the number of CD+4T cells,CD+3T cells and the levels of IL-10 and IL-27 in the spleen tissues,and thus it regulates the immune microenvironment and prevents the occurrence of diabetes.