槲皮素对急性痛风性关节炎大鼠的治疗作用及其对肝功能的影响

Therapeutic effect of quercetin for acute gouty arthritis in rats and its influence on liver function

目的探讨槲皮素对急性痛风性关节炎(AGA)大鼠的治疗作用及其对肝功能的影响。方法将50只大鼠随机分为A^E组,每组10只。A、B组均以蒸馏水灌胃,C^E组分别予100、200、400 mg/kg的槲皮素灌胃,1次/d,连续7 d;第5天给药1 h后B^E组采用微晶尿酸钠和次黄嘌呤法建立AGA模型。造模前及造模后2、6、12、24、48 h测算大鼠右后肢小腿踝关节肿胀率;造模后8、48 h检测血清尿酸水平;造模后48 h检测血清ALT、AST、TBIL、DBIL,计算肝脏指数,HE染色观察肝脏组织病理变化。结果 B^E组各时间点大鼠踝关节肿胀率均较A组升高(P均<0.01),C^E组较B组肿胀率下降(P均<0.01);C^E组两两比较,P均>0.05。B^E组造模后8 h血清尿酸水平均较A组升高(P均<0.01),造模后48 h血尿酸与A组比较差异均无统计学意义;B^E组血清UA水平两两比较,P均>0.05。各组血清ALT、AST、TBIL、DBIL及肝脏指数两两比较,P均>0.05。各组均未发现明显组织病理形态学改变。结论槲皮素可减轻AGA大鼠的关节肿胀,但不降低血尿酸水平,对肝功能无明显影响。

Objective To observe the therapeutic effect of quercetin for acute gouty arthritis( AGA) in rats and its influence on liver function. Methods Fifty rats were randomly divided into groups A-E,and 10 in each group. Rats in the group A and group B were administered with distilled water,and rats in the groups C,D and E were administered with100,200 and 400 mg / kg quercetin,respectively. All rats were administered by oral gavage once a day for seven consecutive days throughout the experiment. On the fifth day,we established AGA models in the groups B-E after administration of1 h. The rat right hind ankle swelling rate was measured at 0,2,6,12,24 and 48 h,the serum blood uric acid( UA)was detected at 8 h and 48 h after modeling,the serum ALT,AST,TBIL and DBIL was detected at 48 h after modeling.The liver indexes were calculated and the liver histopathological changes were observed with HE staining. Results The ankle swelling rates of B-E groups at all time points were increased as compared with that in the group A( all P < 0. 01),but the swelling rates of groups C-E were decreased as compared with that of group B( all P < 0. 01),and no significant difference was found in the swelling rate among groups C-E( all P > 0. 05). The serum UA levels of groups B-E after modeling8 h were elevated as compared with that of the group A( all P < 0. 01),and there was no significant differences between group A and groups B-E after modeling 48 h( P > 0. 05); meanwhile,the serum UA was not significantly different between groups B-E( all P > 0. 05). No significant differences were found in the serum ALT,AST,TBIL,DBIL and liver indexes between each two groups( all P > 0. 05). Furthermore,no significant pathological changes were found in each group. Conclusion Quercetin can reduce the joint swelling in AGA rats,but do not decrease the level of serum UA and has no significant effect on liver function.